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验证数据展示

  • WB analysis using 68427-1-Ig

    WB analysis using 68427-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68427-1-Ig (HERPUD2 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IF Staining of HepG2 using 68427-1-Ig

    IF Staining of HepG2 using 68427-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using HERPUD2 antibody (68427-1-Ig, Clone: 1H10H1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

HERPUD2 Monoclonal antibody

HERPUD2 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68427-1-Ig

产品说明书

CloneNo. 1H10H1

宿主/亚型

Mouse / IgG3

种属反应性

Human

应用

WB, IF/ICC, ELISA

别名

FLJ22313, FLJ31032, HERPUD family member 2, HERPUD2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inNCI-H1299 cells, L02 clls, HepG2 cells, A549 cells, HT-29 cells, LNCaP cells, K-562 cells
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68427-1-Ig targets HERPUD2 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human
免疫原 HERPUD2 fusion protein Ag15007 种属同源性预测
宿主/亚型 Mouse / IgG3
抗体类别 Monoclonal
产品类型 Antibody
全称 HERPUD family member 2
别名 FLJ22313, FLJ31032, HERPUD family member 2, HERPUD2
计算分子量 406 aa, 45 kDa
观测分子量35-45 kDa
GenBank蛋白编号BC005091
基因名称 HERPUD2
Gene ID (NCBI) 64224
RRIDAB_3085141
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9BSE4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HERPUD2, also named as Homocysteine-responsive endoplasmic reticulum-resident ubiquitin-like domain member 2 protein, is a 406 amino acid protein, which contains one ubiquitin-like domain. HERPUD2 as a single-pass membrane protein could be involved in the unfolded protein response (UPR) pathway. HERPUD2 may exstis as two isoforms 406aa,45 kDa and 297aa 33 kDa (Genebank:EAW94050).

实验方案

Product Specific Protocols
WB protocol for HERPUD2 antibody 68427-1-IgDownload protocol
IF protocol for HERPUD2 antibody 68427-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols