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验证数据展示

  • WB analysis using 68260-1-Ig

    WB analysis using 68260-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68260-1-Ig (SNX12 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IF Staining of HeLa using 68260-1-Ig

    IF Staining of HeLa using 68260-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SNX12 antibody (68260-1-Ig, Clone: 2C6C8 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-phalloidin (red).

SNX12 Monoclonal antibody

SNX12 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68260-1-Ig

产品说明书

CloneNo. 2C6C8

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

Sorting nexin-12, 2C6C8

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, MCF-7 cells, HeLa cells, HepG2 cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68260-1-Ig targets SNX12 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SNX12 fusion protein Ag3014 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 sorting nexin 12
别名 Sorting nexin-12, 2C6C8
计算分子量 172 aa, 20 kDa
观测分子量18 kDa
GenBank蛋白编号BC020559
基因名称 SNX12
Gene ID (NCBI) 29934
RRIDAB_2935345
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9UMY4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SNXs (sorting nexins) are a diverse group of cytoplasmic and membrane-associated proteins that are classified by the presence of a phospholipid-binding motif-the PX domain (PMID:12461558). SNXs are involved in endocytosis and protein trafficking. SNX12 (Sorting nexin 12) is localized in early endosomes and SNX12 overexpression specifically prevents the degradative pathway from early to late endosomes/lysosomes (PMID: 22719997). SNX12 may participate in Alzheimer's disease (AD) pathology by regulating the endocytosis of BACE1 and affecting β-processing of APP (PMID: 22709416).

实验方案

Product Specific Protocols
WB protocol for SNX12 antibody 68260-1-IgDownload protocol
IF protocol for SNX12 antibody 68260-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols