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验证数据展示

  • WB analysis using 68247-1-Ig

    WB analysis using 68247-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68247-1-Ig (KLK8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of EC109 using 68247-1-Ig

    WB analysis of EC109 using 68247-1-Ig

    EC109 cells were subjected to SDS PAGE followed by western blot with 68247-1-Ig (KLK8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of A431 using 68247-1-Ig

    WB analysis of A431 using 68247-1-Ig

    A431 cells were subjected to SDS PAGE followed by western blot with 68247-1-Ig (KLK8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human cervical cancer using 68247-1-Ig

    IHC staining of human cervical cancer using 68247-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 68247-1-Ig (KLK8 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KLK8 antibody included
  • IHC staining of human cervical cancer using 68247-1-Ig

    IHC staining of human cervical cancer using 68247-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 68247-1-Ig (KLK8 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KLK8 antibody included
  • IHC staining of mouse brain using 68247-1-Ig

    IHC staining of mouse brain using 68247-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68247-1-Ig (KLK8 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KLK8 antibody included
  • IHC staining of mouse brain using 68247-1-Ig

    IHC staining of mouse brain using 68247-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68247-1-Ig (KLK8 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KLK8 antibody included
  • IF Staining of SH-SY5Y using 68247-1-Ig

    IF Staining of SH-SY5Y using 68247-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using KLK8 antibody (68247-1-Ig, Clone: 2F3A8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of SH-SY5Y using 68247-1-Ig

    IF Staining of SH-SY5Y using 68247-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using KLK8 antibody (68247-1-Ig, Clone: 2F3A8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

KLK8 Monoclonal antibody

KLK8 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 68247-1-Ig

产品说明书

CloneNo. 2F3A8

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

Kallikrein 8, Kallikrein-8, Kallikrein8, kallikrein related peptidase 8, EC:3.4.21.118

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inJurkat cells, A431 cells, EC109 cells, MOLT-4 cells, NK-92 cells
Positive IHC detected inhuman cervical cancer tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells
Planning an IHC experiment? We recommend our IHCeasy KLK8 Ready-To-Use IHC Kit. KLK8 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68247-1-Ig targets KLK8 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag5642

Product name: Recombinant human KLK8 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-260 aa of BC040887

Sequence: MGRPRPRAAKTWMFLLLLGGAWAGHSRAQEDKVLGGHECQPHSQPWQAALFQGQQLLCGGVLVGGNWVLTAAHCKKPKYTVRLGDHSLQNKDGPEQEIPVVQSIPHPCYNSSDVEDHNHDLMLLQLRDQASLGSKVKPISLADHCTQPGQKCTVSGWGTVTSPRENFPDTLNCAEVKIFPQKKCEDAYPGQITDVMVCAGSSKGADTCQGDSGGPLVCDGALQGITSWGSDPCGRSDKPGVYTNICRYLDWIKKIIGSKG

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 kallikrein-related peptidase 8
别名 Kallikrein 8, Kallikrein-8, Kallikrein8, kallikrein related peptidase 8, EC:3.4.21.118
计算分子量 28 kDa
观测分子量28 kDa
GenBank蛋白编号BC040887
基因名称 KLK8
Gene ID (NCBI) 11202
RRIDAB_2935333
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO60259
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KLK8(Kallikrein-8) is also named as NRPN, PRSS19, TADG14.It is a new member of the human kallikrein family, which is predicted to be secreted and expected to be present in biological fluids (PMID:12507964).The protein is detectable in ovarian cancer tissue extracts, serum, and ascites fluid, indicating that it may serve as a new ovarian cancer marker (PMID:12782581). KLK8 has one predicted glycosylation site (PMID:20940292) and it has some isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
IF protocol for KLK8 antibody 68247-1-IgDownload protocol
IHC protocol for KLK8 antibody 68247-1-IgDownload protocol
WB protocol for KLK8 antibody 68247-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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