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验证数据展示

  • WB analysis using 68010-1-Ig

    WB analysis using 68010-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 68010-1-Ig (GMEB2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • WB analysis using 68010-1-Ig

    WB analysis using 68010-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68010-1-Ig (GMEB2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • WB analysis using 68010-1-Ig

    WB analysis using 68010-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68010-1-Ig (GMEB2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of MCF-7 using 68010-1-Ig

    WB analysis of MCF-7 using 68010-1-Ig

    WB result of GMEB2 antibody (68010-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GMEB2 transfected MCF-7 cells.

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  • KD/KO验证

GMEB2 Monoclonal antibody

GMEB2 Monoclonal Antibody for WB, ELISA
Cat No. 68010-1-Ig

产品说明书

CloneNo. 2H10H8

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat

应用

WB, ELISA

别名

DNA binding protein p79PIF, GMEB 2, GMEB2, KIAA1269, P79PIF, PIF p79, PIF79

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, MCF-7 cells, Jurkat cells, RAW 264.7 cells, HEK-293 cells, K-562 cells, PC-12 cells, HSC-T6 cells, NIH/3T3 cells
Planning an IHC experiment? We recommend our IHCeasy GMEB2 Ready-To-Use IHC Kit. GMEB2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68010-1-Ig targets GMEB2 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原

CatNo: Ag31393

Product name: Recombinant human GMEB2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-304 aa of BC036305

Sequence: MATPDVSVHMEEVVVVTTPDTAVDGSGVEGVKTVLVTTNLAPHGGDLTEDNMETENAAAAAAAAFTASSQLKEAVLVKMAEEGENLEAEIVYPITCGDSRANLIWRKFVCPGINVKCVQYDEHVISPKEFVHLAGKSTLKDWKRAIRMNGIMLRKIMDSGELDFYQHDKVCSNTCRSTKIDLSGARVSLSSPTSAEYIPLTPAAADVNGSPATITIETCEDPGDWTAAIGDDTFTFWRGLKDAGLLDEVIQEFHQELVETMRGLQQRVQDPPLQLRDAVLLNNIVQNFGMLDLVKKVLASHKCQ

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 glucocorticoid modulatory element binding protein 2
别名 DNA binding protein p79PIF, GMEB 2, GMEB2, KIAA1269, P79PIF, PIF p79, PIF79
计算分子量 530 aa, 56 kDa
观测分子量68 kDa
GenBank蛋白编号BC036305
基因名称 GMEB2
Gene ID (NCBI) 26205
RRIDAB_2918757
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ9UKD1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GMEB-2 (glucocorticoid modulatory element-binding protein 2), also known as PIF79 (Parvovirus initiation factor p79) or P79PIF, is a DNA-binding protein that plays a crucial role in modulating transcription upon activation by steroid hormones. GMEB-2 is ubiquitously expressed with preferential expression in developmentally important tissues.

实验方案

Product Specific Protocols
WB protocol for GMEB2 antibody 68010-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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