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验证数据展示

  • WB analysis of BxPC-3 using 67954-1-Ig

    WB analysis of BxPC-3 using 67954-1-Ig

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 67954-1-Ig (RON, MST1R antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using 67954-1-Ig

    FC experiment of HeLa using 67954-1-Ig

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug RON, MST1R Monoclonal antibody (67954-1-Ig, Clone:2D8D1) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

RON, MST1R Monoclonal antibody

RON, MST1R Monoclonal Antibody for WB, ELISA
Cat No. 67954-1-Ig

产品说明书

CloneNo. 2D8D1

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, ELISA

别名

MST1R, Macrophage-stimulating protein receptor alpha chain, Macrophage-stimulating protein receptor, EC:2.7.10.1, CDw136

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inBxPC-3 cells, HeLa cells, PC-3 cells
Positive FC (Intra) detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67954-1-Ig targets RON, MST1R in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 RON, MST1R fusion protein Ag28289 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 macrophage stimulating 1 receptor (c-met-related tyrosine kinase)
别名 MST1R, Macrophage-stimulating protein receptor alpha chain, Macrophage-stimulating protein receptor, EC:2.7.10.1, CDw136
计算分子量 152 kDa
观测分子量150-185 kDa
GenBank蛋白编号BQ936677 NM_002447
基因名称 RON
Gene ID (NCBI) 4486
RRIDAB_2935253
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ04912
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RON, MST1R antibody 67954-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols