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验证数据展示

  • WB analysis using 66644-1-Ig

    WB analysis using 66644-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66644-1-Ig (IGFBP2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of A549 using 66644-1-Ig

    IF Staining of A549 using 66644-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using IGFBP2 antibody (66644-1-Ig, Clone: 2F10D1 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

IGFBP2 Monoclonal antibody

IGFBP2 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 66644-1-Ig

产品说明书

CloneNo. 2F10D1
引用文献(1)

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA and More (1)

别名

IBP-2, IBP2, IBP 2, BP2, BP 2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells, T-27D cells, MCF-7 cells, PC-3 cells, NCCIT cells, 4T1 cells
Positive IF/ICC detected inA549 cells
Planning an IHC experiment? We recommend our IHCeasy IGFBP2 Ready-To-Use IHC Kit. IGFBP2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

66644-1-Ig targets IGFBP2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用IHC
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原

CatNo: Ag8421

Product name: Recombinant human IGFBP2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 76-328 aa of BC004312

Sequence: MPCAELVREPGCGCCSVCARLEGEACGVYTPRCGQGLRCYPHPGSELPLQALVMGEGTCEKRRDAEYGASPEQVADNGDDHSEGGLVENHVDSTMNMLGGGGSAGRKPLKSGMKELAVFREKVTEQHRQMGKGGKHHLGLEEPKKLRPPPARTPCQQELDQVLERISTMRLPDERGPLEHLYSLHIPNCDKHGLYNLKQCKMSLNGQRGECWCVNPNTGKLIQGAPTIRGDPECHLFYNEQQEARGVHTQRMQ

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 IGF binding protein 2
别名 IBP-2, IBP2, IBP 2, BP2, BP 2
计算分子量 36 kDa
观测分子量 36 kDa
GenBank蛋白编号BC004312
基因名称 IGFBP2
Gene ID (NCBI) 3485
RRIDAB_2882002
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP18065
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Insulin-like growth factors (IGFs) and their binding proteins (IGFBPs) have important metabolic roles in the human body. The levels of IGFBP2 are elevated during progression of many human cancers.

实验方案

Product Specific Protocols
WB protocol for IGFBP2 antibody 66644-1-IgDownload protocol
IF protocol for IGFBP2 antibody 66644-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
HumanIHC

Cancer Med

The preoperative neutrophil-to-lymphocyte ratio is not a marker of prostate cancer characteristics but is an independent predictor of biochemical recurrence in patients receiving radical prostatectomy.

Authors - Zhi Cao
View Article