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验证数据展示

  • IF Staining of HeLa using 60919-1-Ig

    IF Staining of HeLa using 60919-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Pericentrin antibody (60919-1-Ig, Clone: 1G6F11 ) at dilution of 1:4000 and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-3).

  • FC experiment of Hela using 60919-1-Ig

    FC experiment of Hela using 60919-1-Ig

    1x10^6 Hela cells were intracellularly stained with 0.2 μg Pericentrin Monoclonal antibody (60919-1-Ig, Clone:1G6F11) and Multi-rAb CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L)(RGAM005)(N/A), or 0.2 μg Mouse IgM Isotype Control (65243-1-Ig, Clone: MOPC-104E) (N/A). Cells were fixed with 4% PFA .

Pericentrin Monoclonal antibody

Pericentrin Monoclonal Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 60919-1-Ig

产品说明书

CloneNo. 1G6F11

宿主/亚型

Mouse / IgM

种属反应性

human

应用

IF/ICC, FC (Intra), ELISA

别名

PCNT, Pericentrin B, Pericentrin-B

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHela cells

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:2000-1:8000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.2 μg per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60919-1-Ig targets Pericentrin in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag27248

Product name: Recombinant human PCNT protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 100-200 aa of AK093923

Sequence: MDLEQLQQKQVNDHPPEQCGMFTVSDHPPEQHGMFTVGDHPPEQRGMFTVSDHPPEQHGMFTVSDHPPEQRGMFTISDHQPEQRGMFTVSDHTPEQRGIFTI

种属同源性预测
宿主/亚型 Mouse / IgM
抗体类别 Monoclonal
产品类型 Antibody
全称 pericentrin
别名 PCNT, Pericentrin B, Pericentrin-B
计算分子量 378 kDa
GenBank蛋白编号AK093923
基因名称 Pericentrin
Gene ID (NCBI) 5116
偶联类型 Unconjugated
形式Liquid
纯化方式Caprylic acid/ammonium sulfate precipitation
UNIPROT IDO95613
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Pericentrin (PCNT) is a large, highly conserved centrosomal protein essential for organizing the pericentriolar material (PCM)-the matrix surrounding the centrioles that nucleates and anchors microtubules. It acts as a scaffold that recruits and positions key regulatory complexes, including γ-tubulin ring complexes (γ-TuRCs), protein kinase A (PKA) and dynein, thereby orchestrating microtubule nucleation, spindle assembly and centrosome maturation throughout the cell cycle

实验方案

Product Specific Protocols
IF protocol for Pericentrin antibody 60919-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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