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验证数据展示

  • WB analysis using 60442-2-Ig

    WB analysis using 60442-2-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60442-2-Ig (ATAD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 60442-2-Ig

    WB analysis using 60442-2-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60442-2-Ig (ATAD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

ATAD1 Monoclonal antibody

ATAD1 Monoclonal Antibody for WB, ELISA
Cat No. 60442-2-Ig

产品说明书

CloneNo. 2A2C3

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, rabbit

应用

WB, ELISA

别名

FNP001, EC:7.4.2.-, ATPase family AAA domain-containing protein 1, ATAD 1, AFDC1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inTT cells, rabbit brain tissue, HeLa cells, HEK-293 cells, rat brain tissue, mouse brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60442-2-Ig targets ATAD1 in WB, ELISA applications and shows reactivity with human, mouse, rat, rabbit samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat, rabbit
免疫原

CatNo: Ag10471

Product name: Recombinant human ATAD1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 164-361 aa of BC010868

Sequence: DKWYGESQKLAAAVFSLAIKLQPSIIFIDEIDSFLRNRSSSDHEATAMMKAQFMSLWDGLDTDHSCQVIVMGATNRPQDLDSAIMRRMPTRFHINQPALKQREAILKLILKNENVDRHVDLLEVAQETDGFSGSDLKEMCRDAALLCVREYVNSTSEESHDEDEIRPVQQQDLHRAIEKMKKSKDAAFQNVLTHVCLD

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ATPase family, AAA domain containing 1
别名 FNP001, EC:7.4.2.-, ATPase family AAA domain-containing protein 1, ATAD 1, AFDC1
计算分子量 361 aa, 41 kDa
观测分子量32-37 kDa
GenBank蛋白编号BC010868
基因名称 ATAD1
Gene ID (NCBI) 84896
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ8NBU5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The mitochondrial AAA (ATPase Associated with diverse cellular Activities) protein ATAD1 (in humans; Msp1 in yeast) removes mislocalized membrane proteins, as well as stuck import substrates from the mitochondrial outer membrane, facilitating their re-insertion into their cognate organelles and maintaining mitochondria's protein import capacity.

实验方案

Product Specific Protocols
WB protocol for ATAD1 antibody 60442-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols