验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, SGC-7901 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
30657-1-AP targets PTGIR in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | PTGIR fusion protein Ag30653 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | prostaglandin I2 (prostacyclin) receptor (IP) |
| 别名 | Prostanoid IP receptor, Prostaglandin I2 receptor, Prostacyclin receptor, PRIPR, PGI2 receptor |
| 计算分子量 | 386 aa, 41 kDa |
| 观测分子量 | 41-45kDa |
| GenBank蛋白编号 | BC075814 |
| 基因名称 | PTGIR |
| Gene ID (NCBI) | 5739 |
| RRID | AB_3669746 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P43119 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Prostaglandin I2 receptor (PTGIR), also known as IP, is a seven-transmembrane receptor that binds G proteins leading to activation of adenylyl cyclase and an increase in cAMP formation. PTGIR is involved in insulin secretion in pancreatic beta-cells and in permselectivity in glomerular podocytes.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PTGIR antibody 30657-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |