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验证数据展示

  • WB analysis using 30516-1-AP

    WB analysis using 30516-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30516-1-AP (STAP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis using 30516-1-AP

    WB analysis using 30516-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30516-1-AP (STAP2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

STAP2 Polyclonal antibody

STAP2 Polyclonal Antibody for WB, ELISA
Cat No. 30516-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

BKS, Breast tumor kinase substrate, BRK substrate, Signal-transducing adaptor protein 2, STAP-2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells, LNCaP cells, MCF-7 cells, MDA-MB-231 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30516-1-AP targets STAP2 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 STAP2 fusion protein Ag28818 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 signal transducing adaptor family member 2
别名 BKS, Breast tumor kinase substrate, BRK substrate, Signal-transducing adaptor protein 2, STAP-2
计算分子量 45 kDa
观测分子量50-55 kDa
GenBank蛋白编号BC000795
基因名称 STAP2
Gene ID (NCBI) 55620
RRIDAB_3086344
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UGK3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

STAP2, also known as BKS, belongs to the signal-transducing adaptor protein (STAP) family, The family includes STAP1 and STAP2, is a group of adaptor molecules involved in regulating multiple signaling pathways. The expression level of STAP2 in tubular cells is significantly higher than that in other renal cells, accounting for approximately 70%. In tubular epithelial cells, which are primarily affected by renal injury, STAP2 is mainly localized in the cytoplasm (PMID: 39533293).

实验方案

Product Specific Protocols
WB protocol for STAP2 antibody 30516-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols