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验证数据展示

  • WB analysis using 30452-1-AP

    WB analysis using 30452-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30452-1-AP (ERBB3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

ERBB3 Polyclonal antibody

ERBB3 Polyclonal Antibody for WB, ELISA
Cat No. 30452-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

HER3, erbB3 S, ErbB 3, EC:2.7.10.1, c erbB3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inMCF-7 cells, MDA-MB-453s cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30452-1-AP targets ERBB3 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag30713

Product name: Recombinant human ERBB3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1037-1342 aa of BC082992

Sequence: TLNRPRGSQSLLSPSSGYMPMNQGNLGESCQESAVSGSSERCPRPVSLHPMPRGCLASESSEGHVTGSEAELQEKVSMCRSRSRSRSPRPRGDSAYHSQRHSLLTPVTPLSPPGLEEEDVNGYVMPDTHLKGTPSSREGTLSSVGLSSVLGTEEEDEDEEYEYMNRRRRHSPPHPPRPSSLEELGYEYMDVGSDLSASLGSTQSCPLHPVPIMPTAGTTPDEDYEYMNRQRDGGGPGGDYAAMGACPASEQGYEEMRAFQGPGHQAPHVHYARLKTLRSLEATDSAFDNPDYWHSRLFPKANAQRT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian)
别名 HER3, erbB3 S, ErbB 3, EC:2.7.10.1, c erbB3
观测分子量200 kDa
GenBank蛋白编号BC082992
基因名称 ERBB3
Gene ID (NCBI) 2065
RRIDAB_3086320
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP21860
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

V-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (ErbB-3, HER3) is a member of the EGF receptor tyrosine kinase family including EGFR (HER1), Neu (ErbB-2, HER2), ErbB-3 (HER3), and ErbB-4 (HER4) that are frequently overexpressed in a variety of carcinomas. These family members form either homodimers or heterodimers upon ligand binding to mediate cell growth. ErbB-3 binds and is activated by neuregulins and NTAK. It forms a heterodimer with each of the other ErbB receptors. ErbB-3 is predominantly expressed in epithelial tissues and the brain and is overexpressed in a subset of human mammary tumors.

实验方案

Product Specific Protocols
WB protocol for ERBB3 antibody 30452-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols