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验证数据展示

  • WB analysis using 30434-1-AP

    WB analysis using 30434-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30434-1-AP (PGT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 30434-1-AP

    IHC staining of human stomach cancer using 30434-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 30434-1-AP (PGT antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PGT antibody included

PGT Polyclonal antibody

PGT Polyclonal Antibody for WB, IHC, ELISA
Cat No. 30434-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

SLCO2A1, PHOAR2, OATP2A1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inNIH/3T3 cells, U-251 cells, mouse heart tissue
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PGT Ready-To-Use IHC Kit. PGT primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30434-1-AP targets PGT in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag32275

Product name: Recombinant human PGT protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 416-518 aa of BC041140

Sequence: FFMGCSTPTVAEVYPPSTSSSIHPQSPACRRDCSCPDSIFHPVCGDNGIEYLSPCHAGCSNINMSSATSKQLIYLNCSCVTGGSASAKTGSCPVPCAHFLLPA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 solute carrier organic anion transporter family, member 2A1
别名 SLCO2A1, PHOAR2, OATP2A1
计算分子量 70 kDa
观测分子量 70 kDa
GenBank蛋白编号BC041140
基因名称 PGT
Gene ID (NCBI) 6578
RRIDAB_3086313
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ92959
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Prostaglandin transporter (PGT), also known as SLCO2A1, has been identified as a functional prostaglandin (PG) carrier that mediates either the release of newly-synthesized PGs by cells and/or the uptake of PGs prior to intracellular oxidation. Northern blot analysis revealed that PGT mRNA has broad tissue distribution, suggesting its role in the transport and/ or metabolic clearance of PG in diverse tissues (PMID: 8787677,22331663).

实验方案

Product Specific Protocols
IHC protocol for PGT antibody 30434-1-APDownload protocol
WB protocol for PGT antibody 30434-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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