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验证数据展示

  • IHC staining of mouse testis using 30156-1-AP

    IHC staining of mouse testis using 30156-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30156-1-AP (LRRC50 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 30156-1-AP

    IHC staining of mouse testis using 30156-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30156-1-AP (LRRC50 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse testis using 30156-1-AP

    IF Staining of mouse testis using 30156-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using LRRC50 antibody (30156-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse testis using 30156-1-AP

    IF Staining of mouse testis using 30156-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using LRRC50 antibody (30156-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

LRRC50 Polyclonal antibody

LRRC50 Polyclonal Antibody for IHC, IF-P, ELISA
Cat No. 30156-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse

应用

IHC, IF-P, ELISA

别名

DKFZp434A119, DNAAF1, FLJ25330, LRRC50

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse testis tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30156-1-AP targets LRRC50 in IHC, IF-P, ELISA applications and shows reactivity with Human, mouse samples.

经测试应用 IHC, IF-P, ELISA Application Description
经测试反应性 Human, mouse
免疫原

CatNo: Ag32822

Product name: Recombinant human LRRC50 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 522-680 aa of BC024009

Sequence: FVTELDGTRTEDLETIRLETKETCCIDDLPDLEDDDETGKSLEDQNMCFPKIEVISSLSDDSDPELDYTSLPVLENLPTDTLSNIFAVSKDTSKAARVPFTDIFKKEAKRDSEIRKQDTKSPRPLIQELSDEDPSGQPLMPPTCQRDAAPLTSTGDRDS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 leucine rich repeat containing 50
别名 DKFZp434A119, DNAAF1, FLJ25330, LRRC50
计算分子量 725 aa, 80 kDa
GenBank蛋白编号BC024009
基因名称 LRRC50
Gene ID (NCBI) 123872
RRIDAB_3086249
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8NEP3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LRRC50 is a cilium-specific protein required for the stability of the ciliary architecture, it plays a role in cytoplasmic preassembly of dynein arms.

实验方案

Product Specific Protocols
IF protocol for LRRC50 antibody 30156-1-APDownload protocol
IHC protocol for LRRC50 antibody 30156-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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