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验证数据展示

  • WB analysis using 30080-1-AP

    WB analysis using 30080-1-AP

    SGC-7901 cells were subjected to SDS PAGE followed by western blot with 30080-1-AP (FUCA2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 30080-1-AP

    IF Staining of HepG2 using 30080-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FUCA2 antibody (30080-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

FUCA2 Polyclonal antibody

FUCA2 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 30080-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

Alpha-L-fucosidase 2, Alpha-L-fucoside fucohydrolase 2, EC:3.2.1.51, Plasma alpha-L-fucosidase, PSEC0151

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inSGC-7901 cells
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30080-1-AP targets FUCA2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 FUCA2 fusion protein Ag32467 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 fucosidase, alpha-L- 2, plasma
别名 Alpha-L-fucosidase 2, Alpha-L-fucoside fucohydrolase 2, EC:3.2.1.51, Plasma alpha-L-fucosidase, PSEC0151
计算分子量 54 kDa
观测分子量 54-60 kDa
GenBank蛋白编号BC003060
基因名称 FUCA2
Gene ID (NCBI) 2519
RRIDAB_3086225
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BTY2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Alpha-L-fucosidase 2 (FUCA2) is a gene that codes for a secreted non-lysosomal enzyme and belongs to the glycosyl hydrolase 29 (GH29) family. The enzyme is responsible for the removal of the alpha-1,6-fucose linked to N-acetylglucosamine residue of glycoproteins. Serum FUCA2 is a potential biomarker used for early detection of esophageal squamous cell carcinoma (ESCC) and hepatocellular carcinoma (HCC). FUCA2 has 2 isoforms with the molecular mass of 20 and 54 kDa (PMID: 34935631). Sometimes higher molecular weight around 60 kDa can also be observed, which may be a modified variant of FUCA2.

实验方案

Product Specific Protocols
WB protocol for FUCA2 antibody 30080-1-APDownload protocol
IF protocol for FUCA2 antibody 30080-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols