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验证数据展示

  • IHC staining of mouse brain using 30046-1-AP

    IHC staining of mouse brain using 30046-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30046-1-AP (BAHCC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 30046-1-AP

    IHC staining of mouse brain using 30046-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30046-1-AP (BAHCC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 30046-1-AP

    IHC staining of mouse brain using 30046-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30046-1-AP (BAHCC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat brain using 30046-1-AP

    IHC staining of rat brain using 30046-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 30046-1-AP (BAHCC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat brain using 30046-1-AP

    IHC staining of rat brain using 30046-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 30046-1-AP (BAHCC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse colon using 30046-1-AP

    IHC staining of mouse colon using 30046-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 30046-1-AP (BAHCC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A549 using 30046-1-AP

    IF Staining of A549 using 30046-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using BAHCC1 antibody (30046-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • IHC staining of mouse brain using 30046-1-AP

    IHC staining of mouse brain using 30046-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30046-1-AP (BAHCC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

BAHCC1 Polyclonal antibody

BAHCC1 Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 30046-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse, rat

应用

IHC, IF/ICC, ELISA

别名

BAHCC1, BAHD2, FLJ23058, KIAA1447

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inmouse brain tissue, rat brain tissue, mouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30046-1-AP targets BAHCC1 in IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原

CatNo: Ag32599

Product name: Recombinant human BAHCC1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1518-1614 aa of NM_001291324.1

Sequence: SLGLLCAELRGGSGGEPAKKRSKLERSVYAGLQTASVEKAQCKKSSCQGGLAPSVAHRVAQLKPKVKSKGLPTGLSSFQQKEATPGGRIREKLSRAK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 BAH domain and coiled-coil containing 1
别名 BAHCC1, BAHD2, FLJ23058, KIAA1447
计算分子量280 kDa
观测分子量 280 kDa
GenBank蛋白编号NM_001291324.1
基因名称 BAHCC1
Gene ID (NCBI) 57597
RRIDAB_3086218
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9P281
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BAHCC1 (BAH and coiled-coil domain-containing protein 1) is also named as BAHD2, KIAA1447 and BAH domain-containing protein 2. BAHCC1 is a transcriptional regulator controlling expression of E2F/KLF-dependent cell-cycle and DNA-repair genes. BAHCC1 associates with BRG1-containing remodeling complexes at the promoters of these genes. BAHCC1 silencing leads to decreased cell proliferation and delayed DNA repair. Consequently, BAHCC1 deficiency cooperates with PARP inhibition to induce melanoma cell death (PMID: 37924516). depletion of BAHCC1, or disruption of the BAHCC1BAH-H3K27me3 interaction, causes derepression of H3K27me3-targeted genes that are involved in tumor suppression and cell differentiation, leading to suppression of oncogenesis (PMID: 33139953).

实验方案

Product Specific Protocols
IF protocol for BAHCC1 antibody 30046-1-APDownload protocol
IHC protocol for BAHCC1 antibody 30046-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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