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验证数据展示

  • WB analysis using 30024-1-AP

    WB analysis using 30024-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30024-1-AP (CCDC18 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat colon using 30024-1-AP

    IHC staining of rat colon using 30024-1-AP

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 30024-1-AP (CCDC18 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of U2OS using 30024-1-AP

    IF Staining of U2OS using 30024-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using CCDC18 antibody (30024-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

CCDC18 Polyclonal antibody

CCDC18 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 30024-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, rat

应用

WB, IHC, IF/ICC, ELISA

别名

CCDC18, dJ717I23.1, NY SAR 41, RP4 717I23.1, Sarcoma antigen NY SAR 24

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA431 cells, Daudi cells, HL-60 cells, HeLa cells
Positive IHC detected inrat colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30024-1-AP targets CCDC18 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 Human, rat
免疫原

CatNo: Ag32681

Product name: Recombinant human CCDC18 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 119-468 aa of BC156745

Sequence: MESSSSDYYNKDNEEESLLANVASLRHELKITEWSLQSLGEELSSVSPSENSDYAPNPSRSEKLILDVQPSHPGLLNYSPYENVCKISGSSTDFQKKPRDKMFSSSAPVDQEIKSLREKLNKLRQQNACLVTQNHSLMTKFESIHFELTQSRAKVSMLESAQQQAASVPILEEQIINLEAEVSAQDKVLREAENKLEQSQKMVIEKEQSLQESKEECIKLKVDLLEQTKQGKRAERQRNEALYNAEELSKAFQQYKKKVAEKLEKVQAEEEILERNLTNCEKENKRLQERCGLYKSELEILKEKLRQLKEENNNGKEKLRIMAVKNSEVMAQLTESRQSILKLESELENK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 coiled-coil domain containing 18
别名 CCDC18, dJ717I23.1, NY SAR 41, RP4 717I23.1, Sarcoma antigen NY SAR 24
观测分子量151 kDa
GenBank蛋白编号BC156745
基因名称 CCDC18
Gene ID (NCBI) 343099
RRIDAB_2935502
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5T9S5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CCDC18 (Coiled-coil domain-containing protein 18) is a Protein that in humans is encoded by the CCDC18 gene. CCDC18 has 2 isoforms with the MW of 168 kDa and 151 kDa.

实验方案

Product Specific Protocols
IF protocol for CCDC18 antibody 30024-1-APDownload protocol
IHC protocol for CCDC18 antibody 30024-1-APDownload protocol
WB protocol for CCDC18 antibody 30024-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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