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验证数据展示

  • WB analysis using 29338-1-AP

    WB analysis using 29338-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29338-1-AP (FOXK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of Jurkat using 29338-1-AP

    WB analysis of Jurkat using 29338-1-AP

    WB result of FOXK1 antibody (29338-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FOXK1 transfected Jurkat cells.

  • IP experiment of K-562 using 29338-1-AP

    IP experiment of K-562 using 29338-1-AP

    IP result of anti-FOXK1 (IP:29338-1-AP, 4ug; Detection:29338-1-AP 1:3000) with K-562 cells lysate 1800 ug.

  • IHC staining of human colon cancer using 29338-1-AP

    IHC staining of human colon cancer using 29338-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29338-1-AP (FOXK1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 29338-1-AP

    IHC staining of human colon cancer using 29338-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29338-1-AP (FOXK1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of SW480 using 29338-1-AP

    IF Staining of SW480 using 29338-1-AP

    Immunofluorescent analysis of (4% PFA) fixed SW480 cells using FOXK1 antibody (29338-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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  • KD/KO验证

FOXK1 Polyclonal antibody

FOXK1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 29338-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, IP, ELISA

别名

FOXK1L, MNF

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inJurkat cells, MDA-MB-231 cells, MKN-45 cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSW480 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29338-1-AP targets FOXK1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag28820

Product name: Recombinant human FOXK1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-100 aa of NM_001037165

Sequence: MAEVGEDSGARALLALRSAPCSPVLCAAAAAAAFPAAAPPPAPAQPQPPPGPPPPPPPPLPPGAIAGAGSSGGSSGVSGDSAVAGAAPALVAAAAASVRQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 forkhead box K1
别名 FOXK1L, MNF
计算分子量 75 kDa
观测分子量 90-100 kDa
GenBank蛋白编号NM_001037165
基因名称 FOXK1
Gene ID (NCBI) 221937
RRIDAB_3669682
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP85037
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Forkhead box (FOX) domains bind DNA and consist of 2 loops connecting beta-sheets that flank an alpha-helix, which is a group of highly conserved transcription factors. Forkhead box k1 (FOXK1) also known as MNF, is a transcription factor that belongs to the forkhead family consisting of the winged-helix DNA-binding domain and the N-terminal and C-terminal transcriptional domains(PMID: 15289879, PMID: 27223064). There are two isoforms of FoxK1: FoxK1-α and FoxK1-β. FoxK1-α (90 kDa) is expressed in committed myoblasts and differentiated myotubes, while FoxK1-β (55 kDa) is expressed mainly in quiescent satellite cells(PMID: 9271401).

实验方案

Product Specific Protocols
IF protocol for FOXK1 antibody 29338-1-APDownload protocol
IHC protocol for FOXK1 antibody 29338-1-APDownload protocol
IP protocol for FOXK1 antibody 29338-1-APDownload protocol
WB protocol for FOXK1 antibody 29338-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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