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验证数据展示

  • WB analysis of mouse heart using 27537-1-AP

    WB analysis of mouse heart using 27537-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 27537-1-AP (SYNDIG1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 27537-1-AP

    IHC staining of human brain using 27537-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 27537-1-AP (SYNDIG1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human brain using 27537-1-AP

    IHC staining of human brain using 27537-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 27537-1-AP (SYNDIG1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SYNDIG1 Polyclonal antibody

SYNDIG1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27537-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse

应用

WB, IHC, ELISA and More (1)

别名

C20orf39, Dispanin subfamily C member 2, DSPC2, Synapse differentiation-inducing gene protein 1, SynDIG1, TMEM90B, Transmembrane protein 90B

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse heart tissue
Positive IHC detected inhuman brain tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27537-1-AP targets SYNDIG1 in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 Human, mouse
文献引用反应性mouse
免疫原 SYNDIG1 fusion protein Ag26442 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 20 open reading frame 39
别名 C20orf39, Dispanin subfamily C member 2, DSPC2, Synapse differentiation-inducing gene protein 1, SynDIG1, TMEM90B, Transmembrane protein 90B
观测分子量 29 kDa
GenBank蛋白编号BC030637
基因名称 SYNDIG1
Gene ID (NCBI) 79953
RRIDAB_2880900
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H7V2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SYNDIG1 antibody 27537-1-APDownload protocol
IHC protocol for SYNDIG1 antibody 27537-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Acta Pharmacol Sin

mTOR regulates cocaine-induced behavioural sensitization through the SynDIG1-GluA2 interaction in the nucleus accumbens.

Authors - Hong-Chun Li
View Article
mouseWB,IF

Neuropharmacology

Synapse differentiation-induced gene 1 regulates stress-induced depression through interaction with the AMPA receptor GluA2 subunit of nucleus accumbens in male mice

Authors - Linhong Jiang
View Article