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验证数据展示

  • WB analysis using 27504-1-AP

    WB analysis using 27504-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 27504-1-AP (PSMD8 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IF Staining of U2OS using 27504-1-AP

    IF Staining of U2OS using 27504-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using PSMD8 antibody (27504-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

PSMD8 Polyclonal antibody

PSMD8 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 27504-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, Mouse

应用

WB, IF/ICC, ELISA

别名

HIP6, HYPF, Nin1p, p31, PSMD8, Rpn12, S14

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inNIH/3T3 cells
Positive IF/ICC detected inU2OS cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27504-1-AP targets PSMD8 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human, Mouse
免疫原 PSMD8 fusion protein Ag26567 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 proteasome (prosome, macropain) 26S subunit, non-ATPase, 8
别名 HIP6, HYPF, Nin1p, p31, PSMD8, Rpn12, S14
计算分子量 257 aa, 30 kDa
观测分子量30 kDa
GenBank蛋白编号BC001164
基因名称 PSMD8
Gene ID (NCBI) 5714
RRIDAB_3669605
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP48556
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The Proteasome 26S Subunit, Non-ATPase (PSMD) gene family, which includes the PSMD1 to PSMD14, regulate ubiqutinated protein breakdown in circulation as well as tumorigenesis. Studies have shown that PSMD8 interacts with the sperm adhesin AQN1 to limit polyfertilization. PSMD8 was more expressed in normal skeletal muscle, cardiac muscle, and tongue muscle; among malignant tumors, PSMD8 was expressed in ovarian cancer, testicular cancer, glioma, and melanoma(PMID:37349676).

实验方案

Product Specific Protocols
WB protocol for PSMD8 antibody 27504-1-APDownload protocol
IF protocol for PSMD8 antibody 27504-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols