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验证数据展示

  • IF Staining of mouse brain using 27202-1-AP

    IF Staining of mouse brain using 27202-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using AP4S1 antibody (27202-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IHC staining of mouse brain using 27202-1-AP

    IHC staining of mouse brain using 27202-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27202-1-AP (AP4S1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 27202-1-AP

    IHC staining of mouse brain using 27202-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27202-1-AP (AP4S1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse brain using 27202-1-AP

    IF Staining of mouse brain using 27202-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using AP4S1 antibody (27202-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

AP4S1 Polyclonal antibody

AP4S1 Polyclonal Antibody for IHC, IF-P, ELISA
Cat No. 27202-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IF-P, ELISA

别名

AP 4 complex subunit sigma 1, AP47B, AP4S1, CLA20, CLAPS4, Sigma 1 subunit of AP 4, Sigma 4 adaptin, Sigma4 adaptin

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inmouse brain tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27202-1-AP targets AP4S1 in IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse
免疫原 AP4S1 fusion protein Ag25661 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 adaptor-related protein complex 4, sigma 1 subunit
别名 AP 4 complex subunit sigma 1, AP47B, AP4S1, CLA20, CLAPS4, Sigma 1 subunit of AP 4, Sigma 4 adaptin, Sigma4 adaptin
计算分子量 17 kDa
观测分子量17 kDa
GenBank蛋白编号BC001259
基因名称 AP4S1
Gene ID (NCBI) 11154
RRIDAB_3085936
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y587
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for AP4S1 antibody 27202-1-APDownload protocol
IF protocol for AP4S1 antibody 27202-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols