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验证数据展示

  • WB analysis using 27160-1-AP

    WB analysis using 27160-1-AP

    human plasma tissue were subjected to SDS PAGE followed by western blot with 27160-1-AP (CPN2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 27160-1-AP

    IHC staining of human liver using 27160-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27160-1-AP (CPN2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver using 27160-1-AP

    IHC staining of human liver using 27160-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27160-1-AP (CPN2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CPN2 Polyclonal antibody

CPN2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27160-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

ACBP, Carboxypeptidase N subunit 2, CPN2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman plasma
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27160-1-AP targets CPN2 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 CPN2 fusion protein Ag25801 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 carboxypeptidase N, polypeptide 2
别名 ACBP, Carboxypeptidase N subunit 2, CPN2
计算分子量 545 aa, 61 kDa
观测分子量83-90 kDa
GenBank蛋白编号BC031569
基因名称 CPN2
Gene ID (NCBI) 1370
RRIDAB_3085929
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP22792
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Human carboxypeptidase N (CPN), a member of the CPN/E subfamily of "regulatory" metallo-carboxypeptidases, is an extracellular glycoprotein synthesized in the liver and secreted into the blood, where it controls the activity of vasoactive peptide hormones, growth factors and cytokines by specifically removing C-terminal basic residues. Normally, CPN circulates in blood plasma as a hetero-tetramer consisting of two 83 kDa (CPN2) domains each flanked by a 48 to 55 kDa catalytic (CPN1) domain (PMID:17157876). CPN2 also performs a crucial biological function in the invasion and migration of breast cancer and can be used as a biomarker for effective diagnosis and treatment of breast cancer.

实验方案

Product Specific Protocols
WB protocol for CPN2 antibody 27160-1-APDownload protocol
IHC protocol for CPN2 antibody 27160-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols