验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, human testis tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
26054-1-AP targets TSEN15 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | TSEN15 fusion protein Ag22082 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | tRNA splicing endonuclease 15 homolog (S. cerevisiae) |
| 别名 | tRNA intron endonuclease Sen15, SEN15 homolog, sen15, HsSEN15, C1orf19 |
| 计算分子量 | 171 aa, 19 kDa |
| 观测分子量 | 18-20 kDa |
| GenBank蛋白编号 | BC022030 |
| 基因名称 | TSEN15 |
| Gene ID (NCBI) | 116461 |
| RRID | AB_3669520 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8WW01 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TSEN15 is the non-catalytic subunit of the tRNA-splicing endonuclease complex, which is a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5' and 3' splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini (PMID: 15109492, 27392077).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TSEN15 antibody 26054-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |