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验证数据展示

  • WB analysis using 25812-1-AP

    WB analysis using 25812-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25812-1-AP (A1CF antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse colon using 25812-1-AP

    IHC staining of mouse colon using 25812-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 25812-1-AP (A1CF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse colon using 25812-1-AP

    IHC staining of mouse colon using 25812-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 25812-1-AP (A1CF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

A1CF Polyclonal antibody

A1CF Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25812-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

APOBEC1-stimulating protein, APOBEC1CF, APOBEC1 complementation factor, ACF65, ACF64

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse liver tissue
Positive IHC detected inmouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25812-1-AP targets A1CF in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 A1CF fusion protein Ag22929 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 APOBEC1 complementation factor
别名 APOBEC1-stimulating protein, APOBEC1CF, APOBEC1 complementation factor, ACF65, ACF64
计算分子量 594 aa, 65 kDa
观测分子量65 kDa
GenBank蛋白编号BC054873
基因名称 A1CF
Gene ID (NCBI) 29974
RRIDAB_3669501
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NQ94
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

A1CF, also known as Apobec-1 complementation factor, is an RNA-binding protein that plays a crucial role in the post-transcriptional editing of apolipoprotein B (apoB) mRNA. It is a component of the enzyme complex responsible for converting a CAA codon for glutamine to a UAA stop codon in apoB mRNA, resulting in the production of a truncated protein, apoB48, instead of the full-length apoB100.

实验方案

Product Specific Protocols
WB protocol for A1CF antibody 25812-1-APDownload protocol
IHC protocol for A1CF antibody 25812-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols