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验证数据展示

  • WB analysis of Y79 using 25202-1-AP

    WB analysis of Y79 using 25202-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 25202-1-AP (ANKRD33 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 25202-1-AP

    IHC staining of human testis using 25202-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 25202-1-AP (ANKRD33 Antibody) at dilution of 1:50 (under 40x lens).

ANKRD33 Polyclonal antibody

ANKRD33 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25202-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

ANKRD33, ankyrin repeat domain 33, C12orf7

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inY79 cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25202-1-AP targets ANKRD33 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 ANKRD33 fusion protein Ag18582 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ankyrin repeat domain 33
别名 ANKRD33, ankyrin repeat domain 33, C12orf7
计算分子量 452 aa, 49 kDa
观测分子量 49 kDa
GenBank蛋白编号BC121153
基因名称 ANKRD33
Gene ID (NCBI) 341405
RRIDAB_2879956
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ7Z3H0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ankyrin repeat domain 33 (ANKRD33), also named as C12orf7, belongs to the ankyrin repeat family and is highly expressed in retina. ANKRD33 exists as two isoforms. The isoform 1 molecular weight is 29kDa and isoform 2 is 49kDa. The function of ANKRD33 is still non-known.

实验方案

Product Specific Protocols
WB protocol for ANKRD33 antibody 25202-1-APDownload protocol
IHC protocol for ANKRD33 antibody 25202-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols