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验证数据展示

  • WB analysis using 24936-1-AP

    WB analysis using 24936-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24936-1-AP (ProSAPiP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat brain using 24936-1-AP

    IHC staining of rat brain using 24936-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 24936-1-AP (ProSAPiP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 24936-1-AP

    IHC staining of mouse brain using 24936-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24936-1-AP (ProSAPiP1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 24936-1-AP

    IF Staining of HeLa using 24936-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ProSAPiP1 antibody (24936-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO验证

ProSAPiP1 Polyclonal antibody

ProSAPiP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 24936-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA and More (1)

别名

ProSAPiP 1, ProSAP-interacting protein 1, LZTS3, Leucine zipper putative tumor suppressor 3, KIAA0552

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24936-1-AP targets ProSAPiP1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IP
经测试反应性 human, mouse, rat
文献引用反应性mouse
免疫原 ProSAPiP1 fusion protein Ag20765 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ProSAPiP1 protein
别名 ProSAPiP 1, ProSAP-interacting protein 1, LZTS3, Leucine zipper putative tumor suppressor 3, KIAA0552
计算分子量 72 kDa
观测分子量 75 kDa
GenBank蛋白编号BC038860
基因名称 ProSAPiP1
Gene ID (NCBI) 9762
RRIDAB_2714022
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO60299
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ProSAPiP1 antibody 24936-1-APDownload protocol
IHC protocol for ProSAPiP1 antibody 24936-1-APDownload protocol
IF protocol for ProSAPiP1 antibody 24936-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IP

Neuron

Prosapip1-Dependent Synaptic Adaptations in the Nucleus Accumbens Drive Alcohol Intake, Seeking, and Reward.

Authors - Sophie Laguesse
  • KD Validated
View Article
WB

J Neurosci

Nacc1 Mutation in Mice Models Rare Neurodevelopmental Disorder with Underlying Synaptic Dysfunction

Authors - Mark A Deehan
View Article