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验证数据展示

  • WB analysis of PC-3 using 24459-1-AP

    WB analysis of PC-3 using 24459-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 24459-1-AP (C10orf67 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

C10orf67 Polyclonal antibody

C10orf67 Polyclonal Antibody for WB, ELISA
Cat No. 24459-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

C10orf67, RP11 792P23.2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inPC-3 cells, A549 cells, human testis tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24459-1-AP targets C10orf67 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 C10orf67 fusion protein Ag21465 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 10 open reading frame 67
别名 C10orf67, RP11 792P23.2
计算分子量 184 aa, 21 kDa
观测分子量 18-21 kDa
GenBank蛋白编号BC035732
基因名称 C10orf67
Gene ID (NCBI) 256815
RRIDAB_2879558
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8IYJ2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RNF17 also termed as ring finger protein 17 is a 1623 amino acid protein, which contains 1 ring type zinc finger and 4 tudor domains. RNF17 is specifically expressed in testis. The RING finger motif presents in many ubiquitin E3 ligases. RNF17 interacts with all four members of the Mad family (Mad1, Mxi1, Mad3 and Mad4), which are basic-helix-loop-helix-leucine zipper transcription factors of the Myc oncoprotein network. RNF17 is component of the tectonic-like complex, which is required for ciliogenesis and sonic hedgehog/SHH signaling. RNF17 is able to form dimers or polymers both in vitro and in vivo, indicating that it may play a role in the assembly of RNF17 granules. RNF17 encodes a novel key regulator of spermiogenesis.

实验方案

Product Specific Protocols
WB protocol for C10orf67 antibody 24459-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols