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验证数据展示

  • WB analysis of HL-60 using 23520-1-AP

    WB analysis of HL-60 using 23520-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 23520-1-AP (HOXD13 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IF Staining of A549 using 23520-1-AP

    IF Staining of A549 using 23520-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed A549 cells using 23520-1-AP (HOXD13 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

HOXD13 Polyclonal antibody

HOXD13 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 23520-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

BDE, BDSD, homeobox D13, Homeobox protein Hox 4I, Homeobox protein Hox D13, HOX4I, HOXD13, SPD

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHL-60 cells, A549 cells
Positive IF/ICC detected inA549 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23520-1-AP targets HOXD13 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 HOXD13 fusion protein Ag20093 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 homeobox D13
别名 BDE, BDSD, homeobox D13, Homeobox protein Hox 4I, Homeobox protein Hox D13, HOX4I, HOXD13, SPD
计算分子量 343 aa, 36 kDa
观测分子量 36 kDa
GenBank蛋白编号BC148863
基因名称 HOXD13
Gene ID (NCBI) 3239
RRIDAB_2879291
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP35453
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HOXD13 antibody 23520-1-APDownload protocol
IF protocol for HOXD13 antibody 23520-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols