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验证数据展示

  • WB analysis using 22324-1-AP

    WB analysis using 22324-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22324-1-AP (OVGP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse ovary using 22324-1-AP

    IHC staining of mouse ovary using 22324-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 22324-1-AP (OVGP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

OVGP1 Polyclonal antibody

OVGP1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 22324-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

CHIT5, EGP, MUC9, Mucin 9, OGP, OVGP1, Oviduct specific glycoprotein, Oviductal glycoprotein, Oviductin

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman placenta tissue, mouse ovary tissue, mouse uterus tissue
Positive IHC detected inmouse ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22324-1-AP targets OVGP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag17771

Product name: Recombinant human OVGP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 329-678 aa of BC126177

Sequence: VGYDNAISFSYKAWFIRREHFGGAMVWTLDMDDVRGTFCGTGPFPLVYVLNDILVRAEFSSTSLPQFWLSSAVNSSSTDPERLAVTTAWTTDSKILPPGGEAGVTEIHGKCENMTITPRGTTVTPTKETVSLGKHTVALGEKTEITGAMTMTSVGHQSMTPGEKALTPVGHQSVTTGQKTLTSVGYQSVTPGEKTLTPVGHQSVTPVSHQSVSPGGTTMTPVHFQTETLRQNTVAPRRKAVAREKVTVPSRNISVTPEGQTMPLRGENLTSEVGTHPRMGNLGLQMEAENRMMLSSSPVIQLPEQTPLAFDNRFVPIYGNHSSVNSVTPQTSPLSLKKEIPENSAVDEEA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 oviductal glycoprotein 1, 120kDa
别名 CHIT5, EGP, MUC9, Mucin 9, OGP, OVGP1, Oviduct specific glycoprotein, Oviductal glycoprotein, Oviductin
计算分子量 678 aa, 75 kDa
观测分子量70 kDa
GenBank蛋白编号BC126177
基因名称 OVGP1
Gene ID (NCBI) 5016
RRIDAB_3085696
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen Affinity purified
UNIPROT IDQ12889
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

OVGP1 (oviduct-specific glycoprotein, also known as MUC9) is a high molecular-weight glycoprotein thought to be exclusively secreted by the non-ciliated epithelial cells of the fallopian tube (PMID: 12444068). Identified first as an estrogen-induced secretory protein in the oviduct, OVGP1 aids in sperm capacitation, fertilization, and early embryonic development (PMID: 28883914). OVGP1 is the major non-serum protein present in OF (oviduct fluid) and its biological activity differs among species (PMID: 27601270).

实验方案

Product Specific Protocols
IHC protocol for OVGP1 antibody 22324-1-APDownload protocol
WB protocol for OVGP1 antibody 22324-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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