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验证数据展示

  • WB analysis of human testis using 22257-1-AP

    WB analysis of human testis using 22257-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 22257-1-AP (TSPY3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 22257-1-AP

    IHC staining of human testis using 22257-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 22257-1-AP (TSPY3 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of rat testis using 22257-1-AP

    IHC staining of rat testis using 22257-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 22257-1-AP (TSPY3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TSPY3 Polyclonal antibody

TSPY3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 22257-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IHC, ELISA

别名

Cancer/testis antigen 78, CT78, TSPY, TSPY1, TSPY3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman testis tissue, LNCaP cells
Positive IHC detected inhuman testis tissue, rat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22257-1-AP targets TSPY3 in WB, IHC, ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, rat
免疫原 TSPY3 fusion protein Ag17762 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 testis specific protein, Y-linked 3
别名 Cancer/testis antigen 78, CT78, TSPY, TSPY1, TSPY3
计算分子量 308 aa, 35 kDa
观测分子量 39-40 kDa
GenBank蛋白编号BC075016
基因名称 TSPY3
Gene ID (NCBI) 728137
RRIDAB_11182707
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP0CV98
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TSPY3 antibody 22257-1-APDownload protocol
IHC protocol for TSPY3 antibody 22257-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols