• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 22184-1-AP

    WB analysis using 22184-1-AP

    mouse heart tissue was subjected to SDS PAGE followed by western blot with 22184-1-AP (Connexin 45 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 22184-1-AP

    IHC staining of mouse brain using 22184-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22184-1-AP (Connexin 45 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

Connexin 45 Polyclonal antibody

Connexin 45 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 22184-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (1)

应用

WB, IHC, ELISA

别名

Connexin-45, Cx45, Gap junction alpha-7 protein, Gap junction gamma-1 protein, GJA7

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inmouse heart tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

22184-1-AP targets Connexin 45 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性rat
免疫原 Connexin 45 fusion protein Ag16742 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 gap junction protein, gamma 1, 45kDa
别名 Connexin-45, Cx45, Gap junction alpha-7 protein, Gap junction gamma-1 protein, GJA7
计算分子量 396 aa, 45 kDa
观测分子量45 kDa
GenBank蛋白编号BC096213
基因名称 Connexin-45
Gene ID (NCBI) 10052
RRIDAB_3085686
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP36383
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Connexin 45 (Cx45), also known as Gap junction gamma protein (GJC1), belongs to the connexin family. Connexins are membrane-spanning proteins that assemble to form gap junction channels that facilitate the transfer of ions and small molecules between cells. A loss-of-function mutation in Cx45 contributing to Atrial fibrillation (AF) and other cardiac arrhythmias (PMID: 33429106).

实验方案

Product Specific Protocols
WB protocol for Connexin 45 antibody 22184-1-APDownload protocol
IHC protocol for Connexin 45 antibody 22184-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Appl Biochem Biotechnol

Cardiac Fibroblasts Enhance MMP2 Activity to Suppress Gap Junction Function in Cardiomyocytes

Authors - Xue Bai
View Article