验证数据展示
经过测试的应用
| Positive WB detected in | COS-7 cells, A549 cells, HEK-293 cells, MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
22161-1-AP targets ZNF154 in WB, ELISA applications and shows reactivity with human, monkey samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, monkey |
| 免疫原 | ZNF154 fusion protein Ag17812 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | zinc finger protein 154 |
| 别名 | KIAA2003, pHZ 92, zinc finger protein 154, ZNF154 |
| 计算分子量 | 437 aa, 50 kDa |
| 观测分子量 | 55-60 kDa |
| GenBank蛋白编号 | BC152404 |
| 基因名称 | ZNF154 |
| Gene ID (NCBI) | 7710 |
| RRID | AB_2879012 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q13106 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Sites that display recurrent, aberrant DNA methylation in cancer represent potential biomarkers for screening and diagnostics. Previously, The hypermethylation at the ZNF154 CpG island in 15 solid epithelial tumor types from 13 different organs. The magnitude and reproducibility of the ZNF154 hypermethylation signal across five solid tumor types reinforces the potential of this site as a biomarker for circulating tumor DNA (ctDNA)(PMID: 26857064 ). The calculated molecular weight of ZNF154 is 50 kDa, but the modified ZNF154 protein is about 55-60 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ZNF154 antibody 22161-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
