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验证数据展示

  • WB analysis of HeLa using 21312-1-AP

    WB analysis of HeLa using 21312-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 21312-1-AP (DEPDC6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human thyroid cancer using 21312-1-AP

    IHC staining of human thyroid cancer using 21312-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer slide using 21312-1-AP (DEPDC6 Antibody) at dilution of 1:50.

DEPDC6 Polyclonal antibody

DEPDC6 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 21312-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

DEP domain containing 6, DEP.6, DEPDC6, Deptor

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, COLO 320 cells, HT-1080 cells
Positive IHC detected inhuman thyroid cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21312-1-AP targets DEPDC6 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag15959

Product name: Recombinant human DEPDC6 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 60-409 aa of BC024746

Sequence: YPNCFVAKELIDWLIEHKEASDRETAIKLMQKLADRGIIHHVCDEHKEFKDVKLFYRFRKDDGTFPLDNEVKAFMRGQRLYEKLMSPENTLLQPREEEGVKYERTFMASEFLDWLVQEGEATTRKEAEQLCHRLMEHGIIQHVSSKHPFVDSNLLYQFRMNFRRRRRLMELLNEKSPSSQETHDSPFCLRKQSHDNRKSTSFMSVSPSKEIKIVSAVRRSSMSSCGSSGYFSSSPTLSSSPPVLCNPKSVLKRPVTSEELLTPGAPYARKTFTIVGDAVGWGFVVRGSKPCHIQAVDPSGPAAAAGMKVCQFVVSVNGLNVLHVDYRTVSNLILTGPRTIVMEVMEELEC

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 DEP domain containing 6
别名 DEP domain containing 6, DEP.6, DEPDC6, Deptor
计算分子量 409 aa, 46 kDa
观测分子量 55 kDa
GenBank蛋白编号BC024746
基因名称 DEPDC6
Gene ID (NCBI) 64798
RRIDAB_2878837
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8TB45
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DEPTOR, also named as DEPDC6, is a negative regulator of the mTORC1 and mTORC2 signaling pathways. Through mTOR interactions, DEPTOR is implicated in pathways for cell growth and differentiation, as well as cell-cycle prolongation or termination. It inhibits the kinase activity of both complexes. The MW will migrate to 50-55 kDa with phospho- form.

实验方案

Product Specific Protocols
IHC protocol for DEPDC6 antibody 21312-1-APDownload protocol
WB protocol for DEPDC6 antibody 21312-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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