验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HepG2 cells, Caco-2 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
19254-1-AP targets COSMC in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | COSMC fusion protein Ag6899 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | C1GALT1-specific chaperone 1 |
| 别名 | C1Gal T2, C1GALT1 chaperone 1, C1GALT1 specific chaperone 1, C1GALT1C1, C1GALT2, c38h2 l1, C38H2 like protein 1, Core 1 beta3 Gal T2, COSMC, HSPC067, MSTP143, UNQ273/PRO310 |
| 计算分子量 | 36 kDa |
| 观测分子量 | 36-37 kDa |
| GenBank蛋白编号 | BC050441 |
| 基因名称 | COSMC |
| Gene ID (NCBI) | 29071 |
| RRID | AB_10638003 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96EU7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
COSMC (C1GALT1C1) is a molecular chaperone required for expression of active T-synthase (CIGALT1) which catalyzes the synthesis of Tn antigen. Tn antigens are cancer-associated glycans and play an important role in tumor progression. Dysfunctional COSMC is able to convert a wild type protein into a tumor-specific antigen affecting tumor cell biology. COSMC overexpression had been observed in various cancers. COSMC mutations have been observed in an autoimmune disease Tn syndrome.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for COSMC antibody 19254-1-AP | Download protocol |
| IHC protocol for COSMC antibody 19254-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Aging Cell Elevated miR-124-3p in the aging colon disrupts mucus barrier and increases susceptibility to colitis by targeting T-synthase. | ||
Nephrol Dial Transplant IgA nephropathy: gut microbiome regulates the production of hypoglycosilated IgA1via the TLR4 signaling pathway | ||
Aging (Albany NY) Cosmc transfection decreases malignant behavior of Tn + cells and enhances sensitivity to apoptosis when induced by Apo2L/TRAIL via alteration of O-glycan structure | ||
Mol Cancer COSMC knockdown mediated aberrant O-glycosylation promotes oncogenic properties in pancreatic cancer. | ||
Biochim Biophys Acta Mol Cell Res SLC35A2 deficiency reduces protein levels of core 1 β-1,3-galactosyltransferase 1 (C1GalT1) and its chaperone Cosmc and affects their subcellular localization | ||
Respir Physiol Neurobiol The Cosmc-mediated effects of neutrophil elastase on T antigen expression in BEAS-2B cells. |
