验证数据展示
经过测试的应用
| Positive IF-P detected in | mouse brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
18156-1-AP targets Piccolo in IF-P, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | IF-P, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 |
CatNo: Ag12952 Product name: Recombinant human PCLO protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-356 aa of BC001304 Sequence: MKKFRVSLVSKVGKQKYVDLNMLSDSENSQHLELHEPPKAVDKAKSPGVDPKQLAAELQKVSLQQSPLVLSSVVEKGSHVHSGPTSAGSSSVPSPGQPGSPSVSKKKHGSSKPTDGTKVVSHPITGEIQLQINYDLGNLIIHILQARNLVPRDNNGYSDPFVKVYLLPGRGAEYKRRTKHVQKSLNPEWNQTVIYKSISMEQLKKKTLEVTVWDYDRFSSNDFLGEVLIDLSSTAHLDNTPRWYPLKEQTESIDHGKSHSSQSSQQSPKPSVIKSRSHGIFPDPSKDMQVPTIEKSHSSPGSSKSSSEGHLRSHGPSRSQSKTSVTQTHLEDAGAAIAAAEAAVQQLRIQPSKRRK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | piccolo (presynaptic cytomatrix protein) |
| 别名 | ACZ, Aczonin, KIAA0559, PCLO, Piccolo, Protein piccolo |
| 计算分子量 | 553 kDa |
| GenBank蛋白编号 | BC001304 |
| 基因名称 | Piccolo |
| Gene ID (NCBI) | 27445 |
| RRID | AB_3085556 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9Y6V0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Piccolo is a presynaptic scaffolding protein of the cytomatrix assembled at the active zone (AZ). AZ is a special region of the presynaptic plasma membrane where synaptic vesicles (SVs) fuse. It has been shown that Piccolo affects presynaptic function by negatively regulating SV exocytosis (PMID: 18519737).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for Piccolo antibody 18156-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
