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验证数据展示

  • IHC staining of human tonsillitis using 17558-1-AP

    IHC staining of human tonsillitis using 17558-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17558-1-AP (WDFY4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 17558-1-AP

    IHC staining of human tonsillitis using 17558-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17558-1-AP (WDFY4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

WDFY4 Polyclonal antibody

WDFY4 Polyclonal Antibody for IHC, ELISA
Cat No. 17558-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IHC, ELISA

别名

C10orf64, KIAA1607, WDFY family member 4, WDFY4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inhuman tonsillitis tissue, human prostate cancer tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17558-1-AP targets WDFY4 in IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 WDFY4 fusion protein Ag11720 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 WDFY family member 4
别名 C10orf64, KIAA1607, WDFY family member 4, WDFY4
计算分子量 354 kDa
观测分子量 ~70 kDa
GenBank蛋白编号BC032420
基因名称 WDFY4
Gene ID (NCBI) 57705
RRIDAB_2288447
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6ZS81
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

WDFY4, also named as C10orf64 and KIAA1607, is a huge protein with unknown functions. WDFY4 is expressed predominantly in immune tissues such as lymph nodes, the spleen, the thymus and the tonsils. According to recent genome-wide association studies (GWAS), WDFY4 was introduced as novel predisposing genes for SLE (Systemic Lupus Erythematosus) which is a heterogeneous complex relapsing-remitting autoimmune disease. This antibody detects the C-terminal of WDFY4. The gene WDFY4 has some isoforms, 354 kDa, 73 kDa, 115 kDa, 353 kDa, 325 kDa( from UNIPROT) and 1992aa, 222 kDa; 3224aa, 358kDa; 624aa, 69 kDa; 1270aa, 143 kDa; 1887aa, 211 kDa (From NCBI).

实验方案

Product Specific Protocols
IHC protocol for WDFY4 antibody 17558-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols