验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, A549 cells, Jurkat cells, HeLa cells |
| Positive IP detected in | MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15529-1-AP targets MTX1 in WB, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB, IP |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag7685 Product name: Recombinant human MTX1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 75-435 aa of BC001906 Sequence: YVGHLWMGRRPPSPEARGPVPRSSAASRARRSLASPGISPGPLTATIGGAVAGGGPRQGRAEAHKEVFPGQRVGKMAAPMELFCWSGGWGLPSVDLDSLAVLTYARFTGAPLKVHKISNPWQSPSGTLPALRTSHGEVISVPHKIITHLRKEVHTFWIDTKNYVEVTRKWYAEAMPFPLNFFLPGRMQRQYMERLQLLTGEHRPEDEEELEKELYREARECLTLLSQRLGSQKFFFGDAPASLDAFVFSYLALLLQAKLPSGKLQVHLRGLHNLCAYCTHILSLYFPWDGAEVPPQRQTPAGPETEEEPYRRRNQILSVLAGLAAMVGYALLSGIVSIQRATPARAPGTRTLGMAEEDEEE 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | metaxin 1 |
| 别名 | metaxin 1, MTX, MTX1, MTXN |
| 计算分子量 | 51 kDa |
| 观测分子量 | 35 kDa |
| GenBank蛋白编号 | BC001906 |
| 基因名称 | MTX1 |
| Gene ID (NCBI) | 4580 |
| RRID | AB_2148416 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q13505 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
METAXINs are proteins located in the outer membrane of the mammalian mitochondrion. METAXIN 1 was characterized firstly and was found to play a role in the import of mitochondrial precursor proteins. It may also play a role in the assembly of b-barrel proteins into the outer membrane METAXIN1 was reported to be essential for survival of the postimplantation mouse embryo. METAXIN 1 has multiple transcripts. Several isoforms of METAXIN 1 exist due to the alternative splicing, with the predicted MW of 51, 48 and 36 kDa, respectively. Metaxin 1 interacts with metaxin 2, a novel related protein associated with the mammalian mitochondrial outer membrane and form complex in the mitochondrion outer membrane.
实验方案
| Product Specific Protocols | |
|---|---|
| IP protocol for MTX1 antibody 15529-1-AP | Download protocol |
| WB protocol for MTX1 antibody 15529-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Int J Biol Sci Genome-scale CRISPRa screening identifies MTX1 as a contributor for sorafenib resistance in hepatocellular carcinoma by augmenting autophagy.
| ||
Hepatology Loss of Sam50 in hepatocytes induces cardiolipin-dependent mitochondrial membrane remodeling to trigger mtDNA release and liver injury | ||
J Cell Sci Myo19 tethers mitochondria to endoplasmic reticulum-associated actin to promote mitochondrial fission
| ||
NPJ Parkinsons Dis Disruption of axonal transport in Parkinson's disease: the role of pathological α-Syn and AMPK/p38 MAPK signaling |
