验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, L02 cells |
| Positive IHC detected in | human placenta tissue, human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy TNFAIP1 Ready-To-Use IHC Kit. TNFAIP1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:300-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 2 publications below |
| WB | See 3 publications below |
产品信息
15320-1-AP targets TNFAIP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | TNFAIP1 fusion protein Ag0220 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | tumor necrosis factor, alpha-induced protein 1 (endothelial) |
| 别名 | Tumor necrosis factor, alpha-induced protein 1, endothelial, EDP1, BTB/POZ domain-containing protein TNFAIP1, BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 2 |
| 计算分子量 | 36 kDa |
| 观测分子量 | 36 kDa |
| GenBank蛋白编号 | BC001949 |
| 基因名称 | TNFAIP1 |
| Gene ID (NCBI) | 7126 |
| RRID | AB_10644288 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q13829 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TNFAIP1 was identified as a human gene activated transcriptionally by tumour necrosis factor alpha in endothelial cells. It is encoded by a 3.5-kilobase transcript and its expression is found to be developmentally regulated in a tissue-specific manner. The open reading frame predicts a 316-amino acid sequence rich in charged residues, particularly at the carboxyl terminus.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TNFAIP1 antibody 15320-1-AP | Download protocol |
| IHC protocol for TNFAIP1 antibody 15320-1-AP | Download protocol |
| IF protocol for TNFAIP1 antibody 15320-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Commun Signal Neuronal-specific TNFAIP1 ablation attenuates postoperative cognitive dysfunction via targeting SNAP25 for K48-linked ubiquitination | ||
Neurotox Res TNFAIP1 Mediates Formaldehyde-Induced Neurotoxicity by Inhibiting the Akt/CREB Pathway in N2a Cells.
| ||
Oncol Lett MicroRNA-373 is upregulated and targets TNFAIP1 in human gastric cancer, contributing to tumorigenesis.
|
