验证数据展示
经过测试的应用
| Positive WB detected in | mouse testis tissue, A375 cells, rat testis tissue |
| Positive IHC detected in | human lung cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A375 cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
| IHC | See 3 publications below |
产品信息
14602-1-AP targets MAGEA6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag6148 Product name: Recombinant human MAGEA6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-314 aa of BC067731 Sequence: MPLEQRSQHCKPEEGLEARGEALGLVGAQAPATEEQEAASSSSTLVEVTLGEVPAAESPDPPQSPQGASSLPTTMNYPLWSQSYEDSSNQEEEGPSTFPDLESEFQAALSRKVAKLVHFLLLKYRAREPVTKAEMLGSVVGNWQYFFPVIFIKASDSLQLVFGIELMEVDPIGHVYIFATCLGLSYDGLLGDNQIMPKTGFLIIILAIIAKEGDCAPEEKIWEELSVLEVFEGREDSIFGDPKKLLTQYFVQENYLEYRQVPGSDPACYEFLWGPRALIETSYVKVLHHMVKISGGPRISYPLLHEWALREGEE 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | melanoma antigen family A, 6 |
| 别名 | Melanoma-associated antigen 6, MAGE-6 antigen, MAGE6, MAGE3B antigen, CT1.6 |
| 计算分子量 | 35 kDa |
| 观测分子量 | 45-50 kDa |
| GenBank蛋白编号 | BC067731 |
| 基因名称 | MAGEA6 |
| Gene ID (NCBI) | 4105 |
| RRID | AB_2250093 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P43360 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for MAGEA6 antibody 14602-1-AP | Download protocol |
| IHC protocol for MAGEA6 antibody 14602-1-AP | Download protocol |
| WB protocol for MAGEA6 antibody 14602-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Exp Neurol Helios modulates the maturation of a CA1 neuronal subpopulation required for spatial memory formation. | ||
Anticancer Res Tissue Expression of Melanoma-associated Antigen A6 and Clinical Characteristics of Gastric Cancer. | ||
Exp Ther Med MAGEA6 positively regulates MSMO1 and promotes the migration and invasion of oesophageal cancer cells.
| ||
Exp Ther Med Investigation into the expression levels of MAGEA6 in esophageal squamous cell carcinoma and esophageal adenocarcinoma tissues. |
