• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 14037-1-AP

    WB analysis using 14037-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14037-1-AP (FEM1C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 14037-1-AP

    IF Staining of HeLa using 14037-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using FEM1C antibody (14037-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human heart using 14037-1-AP

    IHC staining of human heart using 14037-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14037-1-AP (FEM1C Antibody) at dilution of 1:50 (under 40x lens).

FEM1C Polyclonal antibody

FEM1C Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 14037-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

Protein fem-1 homolog C, FEM1-gamma, FEM1A, FEM1 gamma, FEM1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inmouse brain tissue, mouse heart tissue, HepG2 cells, rat brain tissue
Positive IHC detected inhuman heart tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14037-1-AP targets FEM1C in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 FEM1C fusion protein Ag5099 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 fem-1 homolog c (C. elegans)
别名 Protein fem-1 homolog C, FEM1-gamma, FEM1A, FEM1 gamma, FEM1
计算分子量 69 kDa
观测分子量 69 kDa
GenBank蛋白编号BC028369
基因名称 FEM1C
Gene ID (NCBI) 56929
RRIDAB_2878002
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96JP0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Protein fem-1 homolog C (FEM1C) is involved in protein modification and ubiquitination.Probable component of an E3 ubiquitin-protein ligase complex, in which it may act as a substrate recognition subunit.Highly expressed in kidney, cardiac tissue, skeletal muscle and testis. Expressed at lower levels in other tissues, including cartilage.This antibody specifically recognizes the 69kd FEM1C protein.

实验方案

Product Specific Protocols
WB protocol for FEM1C antibody 14037-1-APDownload protocol
IHC protocol for FEM1C antibody 14037-1-APDownload protocol
IF protocol for FEM1C antibody 14037-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols