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验证数据展示

  • IP experiment of HeLa using 13993-1-AP

    IP experiment of HeLa using 13993-1-AP

    IP result of anti-NEDD1 (IP:13993-1-AP, 3ug; Detection:13993-1-AP 1:800) with HeLa cells lysate 2150ug.

  • WB analysis using 13993-1-AP

    WB analysis using 13993-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13993-1-AP (NEDD1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 13993-1-AP

    IHC staining of human liver using 13993-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13993-1-AP (NEDD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NEDD1 antibody included
  • IHC staining of human liver using 13993-1-AP

    IHC staining of human liver using 13993-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13993-1-AP (NEDD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NEDD1 antibody included
  • IHC staining of human colon using 13993-1-AP

    IHC staining of human colon using 13993-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13993-1-AP (NEDD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NEDD1 antibody included
  • IHC staining of human colon using 13993-1-AP

    IHC staining of human colon using 13993-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 13993-1-AP (NEDD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with NEDD1 antibody included

NEDD1 Polyclonal antibody

NEDD1 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 13993-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, IHC, ELISA and More (1)

别名

GCP WD, NEDD 1, NEDD1, Protein NEDD1, TUBGCP7

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, human brain tissue, SH-SY5Y cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman liver tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy NEDD1 Ready-To-Use IHC Kit. NEDD1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13993-1-AP targets NEDD1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, IHC, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原

CatNo: Ag5077

Product name: Recombinant human NEDD1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-332 aa of BC027605

Sequence: MQENLRFASSGDDIKIWDASSMTLVDKFNPHTSPHGISSICWSSNNNFLVTASSSGDKIVVSSCKCKPVPLLELAEGQKQTCVNLNSTSMYLVSGGLNNTVNIWDLKSKRVHRSLKDHKDQVTCVTYNWNDCYIASGSLSGEIILHSVTTNLSSTPFGHGSNQSVRHLKYSLFKKSLLGSVSDNGIVTLWDVNSQSPYHNFDSVHKAPASGICFSPVNELLFVTIGLDKRIILYDTSSKKLVKTLVADTPLTAVDFMPDGATLAIGSSRGKIYQYDLRMLKSPVKTISAHKTSVQCIAFQYSTVLTKSSLNKGCSNKPTTVNKRSVNVNAAS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 neural precursor cell expressed, developmentally down-regulated 1
别名 GCP WD, NEDD 1, NEDD1, Protein NEDD1, TUBGCP7
计算分子量 72 kDa
观测分子量 75 kDa, 62 kDa
GenBank蛋白编号BC027605
基因名称 NEDD1
Gene ID (NCBI) 121441
RRIDAB_2149202
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8NHV4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NEDD1 is required for mitosis progression. NEDD1 promotes the nucleation of microtubules from the spindle. This antibody works well in WB, IHC, IP and IF application.

实验方案

Product Specific Protocols
WB protocol for NEDD1 antibody 13993-1-APDownload protocol
IHC protocol for NEDD1 antibody 13993-1-APDownload protocol
IP protocol for NEDD1 antibody 13993-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Science

The mechanism of acentrosomal spindle assembly in human oocytes

Authors - Tianyu Wu
View Article
humanWB,IHC

J Cancer

NEDD1 Promotes the Development of Lung Adenocarcinoma and Can be Used as a Prognostic Marker

Authors - Zhen Yang
  • KD Validated
View Article