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验证数据展示

  • WB analysis of HeLa using 13888-1-AP

    WB analysis of HeLa using 13888-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 13888-1-AP (NKIAMRE antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human gliomas using 13888-1-AP

    IHC staining of human gliomas using 13888-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13888-1-AP (NKIAMRE antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human gliomas using 13888-1-AP

    IHC staining of human gliomas using 13888-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13888-1-AP (NKIAMRE antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

NKIAMRE Polyclonal antibody

NKIAMRE Polyclonal Antibody for WB, IHC, ELISA
Cat No. 13888-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

CDKL3, cyclin dependent kinase like 3, NKIAMRE

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13888-1-AP targets NKIAMRE in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human
文献引用反应性human
免疫原

CatNo: Ag4812

Product name: Recombinant human CDKL3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-279 aa of BC041799

Sequence: MEMYETLGKVGEGSYGTVMKCKHKNTGQIVAIKIFYERPEQSVNKIAMREIKFLKQFHHENLVNLIEVFRQKKKIHLVFEFIDHTVLDELQHYCHGLESKRLRKYLFQILRAIDYLHSNNIIHRDIKPENILVSQSGITKLCDFGFARTLAAPGDIYTDYVATRWYRAPELVLKDTSYGKPVDIWALGCMIIEMATGNPYLPSSSDLDLLHKIVLKVGNLSPHLQNIFSKSPIFAGVVLPQVQHPKNARKKYPKLNGLLADIVHACLQIDPADRISSSD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cyclin-dependent kinase-like 3
别名 CDKL3, cyclin dependent kinase like 3, NKIAMRE
计算分子量 592 aa, 68 kDa
观测分子量 52-60 kDa
GenBank蛋白编号BC041799
基因名称 NKIAMRE
Gene ID (NCBI) 51265
RRIDAB_2077463
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8IVW4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NKIAMRE is also named as CDKL3(Cyclin-dependent kinase-like 3) and belongs to the protein kinase superfamily.This protein contains the MAPK-like TDY motif for threonine and tyrosine phosphorylation, as well as the putative cyclin-binding motif(PMID:10463609). It has 2 isoforms produced by alternative splicing with the molecular mass of 52 kDa and 68 kDa.

实验方案

Product Specific Protocols
WB protocol for NKIAMRE antibody 13888-1-APDownload protocol
IHC protocol for NKIAMRE antibody 13888-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Front Oncol

CDKL3 Targets ATG5 to Promote Carcinogenesis of Esophageal Squamous Cell Carcinoma.

Authors - Suna Zhou
View Article
humanWB,IHC

Front Physiol

Curcumol Exerts Anticancer Effect in Cholangiocarcinoma Cells via Down-Regulating CDKL3.

Authors - Jinduo Zhang
  • KD Validated
View Article