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验证数据展示

  • WB analysis of mouse testis using 12559-1-AP

    WB analysis of mouse testis using 12559-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 12559-1-AP (STK36 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate using 12559-1-AP

    IHC staining of human prostate using 12559-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate using 12559-1-AP (STK36 antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with STK36 antibody included
  • IHC staining of human prostate using 12559-1-AP

    IHC staining of human prostate using 12559-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate using 12559-1-AP (STK36 antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with STK36 antibody included
  • IP experiment of mouse testis using 12559-1-AP

    IP experiment of mouse testis using 12559-1-AP

    IP result of anti-STK36 (IP:12559-1-AP, 4ug; Detection:12559-1-AP 1:500) with mouse testis tissue lysate 4000ug.

  • IHC staining of human lung cancer using 12559-1-AP

    IHC staining of human lung cancer using 12559-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12559-1-AP (STK36 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with STK36 antibody included
  • IHC staining of human lung cancer using 12559-1-AP

    IHC staining of human lung cancer using 12559-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12559-1-AP (STK36 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with STK36 antibody included
  • IHC staining of mouse stomach using 12559-1-AP

    IHC staining of mouse stomach using 12559-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 12559-1-AP (STK36 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with STK36 antibody included
  • IHC staining of rat stomach using 12559-1-AP

    IHC staining of rat stomach using 12559-1-AP

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 12559-1-AP (STK36 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with STK36 antibody included

STK36 Polyclonal antibody

STK36 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 12559-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, IHC, ELISA and More (1)

别名

FU, Fused homolog, KIAA1278, STK36

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman prostate tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy STK36 Ready-To-Use IHC Kit. STK36 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12559-1-AP targets STK36 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, IHC, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 STK36 fusion protein Ag3279 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 serine/threonine kinase 36, fused homolog (Drosophila)
别名 FU, Fused homolog, KIAA1278, STK36
计算分子量 1315 aa, 144 kDa
观测分子量 144 kDa
GenBank蛋白编号BC026158
基因名称 STK36
Gene ID (NCBI) 27148
RRIDAB_2196785
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NRP7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

STK36(Serine/threonine-protein kinase 36) is also named as KIAA1278 and belongs to the Ser/Thr protein kinase family. The Serine/threonine protein kinase plays an important role in the sonic hedgehog (Shh) pathway by regulating the activity of GLI transcription factors and controls the activity of the transcriptional regulators GLI1, GLI2 and GLI3 by opposing the effect of SUFU and promoting their nuclear localization. It has 2 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for STK36 antibody 12559-1-APDownload protocol
IHC protocol for STK36 antibody 12559-1-APDownload protocol
IP protocol for STK36 antibody 12559-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Dev Biol

Mammalian Fused is essential for sperm head shaping and periaxonemal structure formation during spermatogenesis.

Authors - Yoko Inès Nozawa
View Article
mouseWB,IF

FASEB J

The putative protein kinase Stk36 is essential for ciliogenesis and CSF flow by associating with Ulk4

Authors - Hongye Zhang
View Article
humanWB,IHC

Sci Rep

Serine/threonine kinase 36 induced epithelial-mesenchymal transition promotes docetaxel resistance in prostate cancer

Authors - Tao He
View Article