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验证数据展示

  • WB analysis using 12399-1-AP

    WB analysis using 12399-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12399-1-AP (HGSNAT antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis using 12399-1-AP

    WB analysis using 12399-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12399-1-AP (HGSNAT antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 12399-1-AP

    IHC staining of human lung cancer using 12399-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 12399-1-AP (HGSNAT antibody) at dilution of 1:50 (under 10x lens).

  • IF Staining of HepG2 using 12399-1-AP

    IF Staining of HepG2 using 12399-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 12399-1-AP (HGSNAT antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

HGSNAT Polyclonal antibody

HGSNAT Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 12399-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

Transmembrane protein 76, TMEM76, MPS3C, HGNAT, Heparan-alpha-glucosaminide N-acetyltransferase

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, mouse liver tissue, A549 cells, HeLa cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12399-1-AP targets HGSNAT in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 HGSNAT fusion protein Ag3089 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 heparan-alpha-glucosaminide N-acetyltransferase
别名 Transmembrane protein 76, TMEM76, MPS3C, HGNAT, Heparan-alpha-glucosaminide N-acetyltransferase
计算分子量 206 aa, 22 kDa
观测分子量 70 kDa
GenBank蛋白编号BC012452
基因名称 HGSNAT
Gene ID (NCBI) 138050
RRIDAB_3085393
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ68CP4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HGSNAT antibody 12399-1-APDownload protocol
IHC protocol for HGSNAT antibody 12399-1-APDownload protocol
IF protocol for HGSNAT antibody 12399-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols