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验证数据展示

  • WB analysis using 12325-1-AP

    WB analysis using 12325-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12325-1-AP (SCYL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 12325-1-AP

    IHC staining of mouse brain using 12325-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12325-1-AP (SCYL2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 12325-1-AP

    IHC staining of mouse brain using 12325-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12325-1-AP (SCYL2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SCYL2 Polyclonal antibody

SCYL2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 12325-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA and More (1)

别名

CVAK104, KIAA1360, SCY1 like 2 (S. cerevisiae), SCY1 like protein 2, SCYL2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293T cells, NIH/3T3 cells, mouse uterus tissue, Jurkat cells, HeLa cells, HepG2 cells
Positive IHC detected inmouse brain tissue, mouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12325-1-AP targets SCYL2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 SCYL2 fusion protein Ag2988 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SCY1-like 2 (S. cerevisiae)
别名 CVAK104, KIAA1360, SCY1 like 2 (S. cerevisiae), SCY1 like protein 2, SCYL2
计算分子量 929 aa, 104 kDa
观测分子量 104 kDa
GenBank蛋白编号BC012387
基因名称 SCYL2
Gene ID (NCBI) 55681
RRIDAB_2071019
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6P3W7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SCYL2 antibody 12325-1-APDownload protocol
IHC protocol for SCYL2 antibody 12325-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

J Neurosci

SCYL2 Protects CA3 Pyramidal Neurons from Excitotoxicity during Functional Maturation of the Mouse Hippocampus.

Authors - Sebastien Gingras
View Article
humanWB,IF

J Cell Biochem

Phosphorylated LASS2 inhibits prostate carcinogenesis via negative regulation of Wnt/β-catenin signaling.

Authors - Kuangen Zhang
View Article