验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, A431 cells, mouse skin tissue, human skin tissue, THP-1 cells, U2OS cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11621-1-AP targets NEIL3 in WB, IP, FC, ChIP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IP, FC, ChIP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 | NEIL3 fusion protein Ag2200 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | nei endonuclease VIII-like 3 (E. coli) |
| 别名 | DNA glycosylase FPG2, DNA glycosylase/AP lyase Neil3, Endonuclease 8 like 3, Endonuclease VIII like 3, FGP2, FPG2, hFPG2, hNEI3, Nei like protein 3, NEI3, NEIL3 |
| 计算分子量 | 605 aa, 68 kDa |
| 观测分子量 | 62-68 kDa |
| GenBank蛋白编号 | BC025954 |
| 基因名称 | NEIL3 |
| Gene ID (NCBI) | 55247 |
| RRID | AB_2251229 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8TAT5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NEIL3 belongs to the FPG family. It is a functional DNA glycosylase in vitro and in vivo. NEIL3 plays a role in repairing FapyG in vivo. (PMID:20185759) NEIL3 has AP lyase activity specific for single-stranded DNA.(PMID:19170771). NEIL3 is specifically expressed in brain areas where neurogenesis takes place during development and that its expression is tightly regulated both temporally and spatially (PMID:19426544). Studies have shown that NEIL3 is recruited to the DSB sites through its GRF zinc finger motifs and plays an important role in MMC/cisplatin-ICL repair by promoting the HR step in FA/BRCA pathway.(PMID: 36351389).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NEIL3 antibody 11621-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun C/EBPβ enhances platinum resistance of ovarian cancer cells by reprogramming H3K79 methylation. | ||
Nucleic Acids Res Cooperation of the NEIL3 and Fanconi anemia/BRCA pathways in interstrand crosslink repair.
| ||
EMBO J PrimPol-mediated repriming facilitates replication traverse of DNA interstrand crosslinks. | ||
Sci Rep NEIL3-mediated proteasomal degradation facilitates the repair of cisplatin-induced DNA damage in human cells | ||
DNA Repair (Amst) Release from quiescence stimulates the expression of human NEIL3 under the control of the Ras dependent ERK-MAP kinase pathway. | ||
Cell Rep NEIL3 Repairs Telomere Damage during S Phase to Secure Chromosome Segregation at Mitosis.
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