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验证数据展示

  • IHC staining of mouse liver using 11276-1-AP

    IHC staining of mouse liver using 11276-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11276-1-AP (PPP2R5C Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PPP2R5C antibody included
  • IHC staining of mouse liver using 11276-1-AP

    IHC staining of mouse liver using 11276-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11276-1-AP (PPP2R5C Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PPP2R5C antibody included
  • WB analysis using 11276-1-AP

    WB analysis using 11276-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11276-1-AP (PPP2R5C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

PPP2R5C Polyclonal antibody

PPP2R5C Polyclonal Antibody for WB, IHC, ELISA
Cat No. 11276-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

B56G, KIAA0044, PP2A B subunit isoform B gamma, PPP2R5C, PR61G

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman placenta tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PPP2R5C Ready-To-Use IHC Kit. PPP2R5C primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11276-1-AP targets PPP2R5C in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 PPP2R5C fusion protein Ag1819 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protein phosphatase 2, regulatory subunit B', gamma isoform
别名 B56G, KIAA0044, PP2A B subunit isoform B gamma, PPP2R5C, PR61G
计算分子量 61 kDa
观测分子量 53-61 kDa
GenBank蛋白编号BC016183
基因名称 PPP2R5C
Gene ID (NCBI) 5527
RRIDAB_3669125
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13362
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PPP2R5C antibody 11276-1-APDownload protocol
IHC protocol for PPP2R5C antibody 11276-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols