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验证数据展示

  • WB analysis using 10299-1-AP

    WB analysis using 10299-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10299-1-AP (CD2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 10299-1-AP

    IHC staining of human tonsillitis using 10299-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10299-1-AP (CD2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0.

  • IHC staining of human tonsillitis using 10299-1-AP

    IHC staining of human tonsillitis using 10299-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10299-1-AP (CD2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0.

CD2 Polyclonal antibody

CD2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 10299-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

CD2, CD2 molecule, Erythrocyte receptor, LFA 2, LFA 3 receptor, Rosette receptor, SRBC, T cell surface antigen CD2, T11

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inJurkat cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10299-1-AP targets CD2 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag0423

Product name: Recombinant human CD2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 25-209 aa of BC033583

Sequence: KEITNALETWGALGQDINLDIPSFQMSDDIDDIKWEKTSDKKKIAQFRKEKETFKEKDTYKLFKNGTLKIKHLKTDDQDIYKVSIYDTKGKNVLEKIFDLKIQERVSKPKISWTCINTTLTCEVMNGTDPELNLYQDGKHLKLSQRVITHKWTTSLSAKFKCTAGNKVSKESSVEPVSCPEKGLD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 CD2 molecule
别名 CD2, CD2 molecule, Erythrocyte receptor, LFA 2, LFA 3 receptor, Rosette receptor, SRBC, T cell surface antigen CD2, T11
计算分子量 351 aa, 39 kDa
观测分子量 50-60 kDa
GenBank蛋白编号BC033583
基因名称 CD2
Gene ID (NCBI) 914
ENSEMBL Gene IDENSG00000116824
RRIDAB_2228874
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP06729
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD2 is a cell surface glycoprotein present on a majority of thymocytes, all mature T cells and subset of NK cells but not on B lymphocytes. It is a pan T-cell marker. CD2 interacts with lymphocyte function-associated antigen (LFA-3/CD58) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.

实验方案

Product Specific Protocols
IHC protocol for CD2 antibody 10299-1-APDownload protocol
WB protocol for CD2 antibody 10299-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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