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  • KD/KO Validated

TMED9 Polyclonal antibody

TMED9 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat, pig


IF/ICC, IHC, IP, WB, ELISA and More (1)



Cat No : 21620-1-AP

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Glycoprotein 25L2, GMP25, GP25L2, HSGP25L2G, TMED9


Positive WB detected inHepG2 cells, L02 cells, pig liver tissue, mouse lung tissue, mouse pancreas tissue, mouse brain tissue, mouse liver tissue, rat liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, HEK-293 cells


Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.


21620-1-AP targets TMED9 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications IF/ICC, IHC, IP, WB, ELISA Application Description
Cited ApplicationsWB, IF, IHC, CoIP
Tested Reactivity human, mouse, rat, pig
Cited Reactivityhuman, mouse
Immunogen TMED9 fusion protein Ag13813 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name transmembrane emp24 protein transport domain containing 9
Synonyms Glycoprotein 25L2, GMP25, GP25L2, HSGP25L2G, TMED9
Calculated Molecular Weight 235 aa, 27 kDa
Observed Molecular Weight 24-27 kDa
GenBank Accession NumberBC001123
Gene Symbol TMED9
Gene ID (NCBI) 54732
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.


TMED9 (also named as GMP25, GP25L2 or p25) is a member of the EMP24/GP25L family. TMED9 probably forms hetero-oligomeric complexes with TMED7 (p27), TMED2 (p24), and TMED10 (p23) (PMID: 10359607). Located in the endoplasmic reticulum and the Golgi, TMED9 appears to be involved in vesicular protein trafficking, mainly in the early secretory pathway (PMID: 9472029; 10359607).


Product Specific Protocols
WB protocol for TMED9 antibody 21620-1-APDownload protocol
IHC protocol for TMED9 antibody 21620-1-APDownload protocol
IF protocol for TMED9 antibody 21620-1-APDownload protocol
IP protocol for TMED9 antibody 21620-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




Small Molecule Targets TMED9 and Promotes Lysosomal Degradation to Reverse Proteinopathy.

Authors - Moran Dvela-Levitt


A Translocation Pathway for Vesicle-Mediated Unconventional Protein Secretion.

Authors - Min Zhang
  • KO Validated

Cell Res

A new type of ERGIC-ERES membrane contact mediated by TMED9 and SEC12 is required for autophagosome biogenesis.

Authors - Shulin Li
  • KD Validated

BMC Biol

ACBD3 modulates KDEL receptor interaction with PKA for its trafficking via tubulovesicular carrier.

Authors - Xihua Yue

Mol Biol Cell

The p24-family and COPII subunit SEC24C facilitate the clearance of alpha1-antitrypsin Z from the endoplasmic reticulum to lysosomes

Authors - Benjamin S Roberts


High expression of transmembrane P24 trafficking protein 9 predicts poor prognosis in breast carcinoma.

Authors - Gaoda Ju
  • KD Validated