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CoraLite® Plus 647 Anti-Mouse MHC Class II (I-A/I-E) (M5/114.15.2)

MHC Class II (I-A/I-E) Monoclonal Antibody for FC, IF

Host / Isotype

Rat / IgG2b, kappa

Reactivity

Mouse

Applications

FC, IF

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

M5/114.15.2

Cat No : CL647-65122

Print datasheet

Synonyms

Aalpha, H 2Aa, H2Aa, H2-Aa, Ia 1, Ia1, IAalpha, MHC Class II (I-A/I-E)

验证数据展示

  • FC experiment of mouse splenocytes using CL647-65122

    FC experiment of mouse splenocytes using CL647-65122

    1X10^6 mouse splenocytes were surface stained with 5 ul CoraLite®488 Anti-Mouse CD3 (17A2) (CL488-65077, Clone: 17A2) and CoraLite® Plus 647-conjugated Rat IgG2b isotype control (left) or 5 ul CoraLite® Plus 647 Anti-Mouse MHC Class II (I-A/I-E) (CL647-65122, Clone: M5/114.15.2) (right). Cells were not fixed.

  • IF Staining of mouse splenocytes using CL647-65122

    IF Staining of mouse splenocytes using CL647-65122

    Immunofluorescent analysis of mouse splenocytes using CoraLite® Plus 647-conjugated Anti-Mouse MHC Class II (I-A/I-E) (CL647-65122, Clone: M5/114.15.2) at dilution of 1:400.

经过测试的应用

Positive IF detected inmouse splenocytes
Positive FC detected inmouse splenocytes
For other applications, we recommend the unconjugated version of this antibody, 65122-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:200-1:800
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

产品信息

CL647-65122 targets MHC Class II (I-A/I-E) in IF, FC applications and shows reactivity with Mouse samples.

Tested Applications FC, IF
Tested Reactivity Mouse
Immunogen Activated C57BL/6 mouse spleen cells 种属同源性预测
Host / Isotype Rat / IgG2b, kappa
Class Monoclonal
Type Antibody
Full Name histocompatibility 2, class II antigen A, alpha
Synonyms Aalpha, H 2Aa, H2Aa, H2-Aa, Ia 1, Ia1, IAalpha, MHC Class II (I-A/I-E)
GenBank accession numberBC031711
Gene symbol H2-Aa
UniprotP14434
Gene ID (NCBI) 14960
RRIDAB_2883695
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths650 nm / 665 nm
Form Liquid
Purification Method Affinity purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

Major histocompatibility complex (MHC) class II molecules are heterodimeric transmembrane glycoproteins that are expressed on the surface of antigen-presenting cells such as dendritic cells, macrophages, and B cells. The M5/114.15.2 antibody detects polymorphic determinants present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying the H-2b, H-2d, H-2q, H-2p, H-2r and H-2u haplotypes but not from mice carrying the H-2s or H-2f haplotypes (PMID: 6170707). This antibody recognizes both I-A and I-E subregion-encoded Ia glycoproteins (I-Ab, I-Ad, I-Aq, I-Ed, I-Ek, not I-Af, I-Ak, or I-As). M5/114 mAb is reported to inhibit I-A subregion-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not the H-2f, H-2k, H-2s haplotypes (PMID: 6173436).

实验方案

Product Specific Protocols
IF protocol for CL Plus 647 MHC Class II (I-A/I-E) antibody CL647-65122Download protocol
FC protocol for CL Plus 647 MHC Class II (I-A/I-E) antibody CL647-65122Download protocol
Standard Protocols
Click here to view our Standard Protocols