• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

FlexAble CoraLite® Plus 555 Antibody Labeling Kit for Mouse IgG1

Application

IF, FC, WB

Reactivity

Mouse

Conjugate

CoraLite® Plus 555

Cat No : KFA022

Print Protocol

Validation Data Gallery

  • Immunofluorescence of rat brain tissue: FFPE rat brain tissue sections were stained with anti-NeuN (66836-1-Ig) labeled with FlexAble CoraLite® Plus 647 Kit (KFA023, magenta), anti-TUBB3 (66375-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA022, orange), and CoraLite®488-conjugated GFAP antibody (CL488-60190, green).

    Immunofluorescence of rat brain tissue: FFPE rat brain tissue sections were stained with anti-NeuN (66836-1-Ig) labeled with FlexAble CoraLite® Plus 647 Kit (KFA023, magenta), anti-TUBB3 (66375-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA022, orange), and CoraLite®488-conjugated GFAP antibody (CL488-60190, green).

  • Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-Lamin B1 (66095-1-Ig) labeled with FlexAble CoraLite® 488 Kit (KFA021, green), anti-HSP60 (66041-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA022, red) and anti-GORASP2 (66627-1-Ig) labeled with FlexAble CoraLite® Plus 647 Kit (KFA023, cyan).​ Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

    Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-Lamin B1 (66095-1-Ig) labeled with FlexAble CoraLite® 488 Kit (KFA021, green), anti-HSP60 (66041-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA022, red) and anti-GORASP2 (66627-1-Ig) labeled with FlexAble CoraLite® Plus 647 Kit (KFA023, cyan).​ Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

  • Flow cytometry of PBMCs. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-human CD45 labeled with FlexAble CoraLite® Plus 647 Kit (KFA003) and anti-CD3 (clone UCHT1, 65151-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA022).

    Flow cytometry of PBMCs. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-human CD45 labeled with FlexAble CoraLite® Plus 647 Kit (KFA003) and anti-CD3 (clone UCHT1, 65151-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA022).

  • Flow cytometry of PBMCs. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-human CD45 labeled with FlexAble CoraLite® Plus 647 Kit (KFA003) and anti-CD4 (clone RPA-T4, 65143-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA022).

    Flow cytometry of PBMCs. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-human CD45 labeled with FlexAble CoraLite® Plus 647 Kit (KFA003) and anti-CD4 (clone RPA-T4, 65143-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA022).

  • WB of HEK293 cell lysates: siRNA transfected HEK293 cell lysates were detected with anti-GNA13 (67188-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA022,green) and anti-PCNA (60097-1-Ig) labeled with FlexAble CoraLite® Plus 647 Kit (KFA023, red).

    WB of HEK293 cell lysates: siRNA transfected HEK293 cell lysates were detected with anti-GNA13 (67188-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA022,green) and anti-PCNA (60097-1-Ig) labeled with FlexAble CoraLite® Plus 647 Kit (KFA023, red).

  • WB of HepG2 cell lysates: siRNA transfected HepG2 cell lysates were detected with anti-CCDC111 (29824-1-AP) labeled with FlexAble CoraLite® Plus 647 Kit (KFA003, red) and anti-PCNA (60097-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA022, green).

    WB of HepG2 cell lysates: siRNA transfected HepG2 cell lysates were detected with anti-CCDC111 (29824-1-AP) labeled with FlexAble CoraLite® Plus 647 Kit (KFA003, red) and anti-PCNA (60097-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA022, green).

  • FlexAble Workflow

    FlexAble Workflow

产品介绍

FlexAble CoraLite® Plus 555 Antibody Labeling Kit for Mouse IgG1 is a novel antibody labeling kit that uses an affinity linker to conjugate CoraLite® Plus 555 in any buffer condition to mouse IgG1 primary antibodies from any supplier. One labeling reaction requires only 0.5µg of antibody – regardless of the antibody concentration. Label your mouse IgG1 antibody in<10 min without any additional equipment.


Product name                

FlexAble CoraLite® Plus 555 Antibody Labeling Kit for Mouse IgG1                

Assay type                

Antibody labeling                

Tested applications                

IF, FC, WB                

Species Reactivity                

Mouse IgG1                

Antibody amount per labeling reaction                

0.5 µg antibody                

Conjugate                

CoraLite® Plus 555

Excitation / Emission maxima wavelengths               

554 nm / 570 nm                


Component            10 rxns            50 rxns
           		4×50 rxns            
CoraLite® Plus 550 FlexLinker for Mouse IgG110 μL50 μL4×50 μL
FlexQuencher for Mouse IgG120 μL100 μL4×100 μL
FlexBuffer100 μL500 μL4×500 μL
Manual1 Copy
Protocol Magnet1 Piece


Storage Condition

Store for 1 year at -20°C or for 6 months at +4°C after shipment. Avoid exposure to light.